Analysis of the complete nucleotide sequence of a white spot syndrome virus isolated from pacific white shrimp
- First Online:
- 359 Downloads
The fourth complete genome sequence of the white spot syndrome virus (WSSV) from Pacific white shrimp (Litopenaeus vannamei) isolated from Korea (WSSV-KR) was determined. The genome is composed of 295,884 bp encompassing 515 open reading frames (ORFs) among which 90 showed no sequence homology with any known protein in BLAST searches. The remaining 425 ORFs encode functional proteins including enzymes for nucleic acid metabolism, DNA replication and transcription, and several major structural proteins. Dot plot and linear comparisons of WSSV Korean strain with other WSSV isolates showed overall similarity but with some areas of sequence difference and one large deletion area.
KeywordsWSSV genome Pacific white shrimp
Unable to display preview. Download preview PDF.
- Chen, L., Leu, J.H., Huang, C.J., Chou, C.M., Chen, S.M., Wang, C.H., Lo, C.F., and Kou, G.H. 2002b. Identification of a nucleocapsid protein (VP35) gene of shrimp white spot syndrome virus and characterization of the motif important for targeting VP35 to the nuclei of transfected insect cells. Virology293, 44–53.PubMedCrossRefGoogle Scholar
- Crockford, M. 2008. White spot disease. Australia and New Zealand Standard Diagnostic Procedures, 1–13. Department of Fisheries c/o Department of Agriculture and Food, South Perth.Google Scholar
- Esparza-Leala, H.M., Escobedo-Bonillac, C.M., Casillas-Hernándezb, R., álvarez-Ruíza, P., Portillo-Clarkd, G., Valerio-Garcíac, R.C., Hernández-Lópezc, J., Méndez-Lozanoa, J., Vibanco-Péreze, N., and Magallón-Barajasd, F.J. 2009. Detection of white spot syndrome virus in filtered shrimp-farm water fractions and experimental evaluation of its infectivity in Penaeus (Litopenaeus) vannamei. Aquaculture 292, 16–22.CrossRefGoogle Scholar
- Jang, I., Suriakala, K., Kim, B., and Meng, X. 2008. Real-Times PCR quantification of white spot syndrome virus (WSSV) and hepatopancreatic parvovirus (HPV) loads in shrimp and seawaters of shrimp ponds on the west coast of South Korea. Fish. Aquat. Sci.11, 195–204.Google Scholar
- Lightner, D.V. and Lo, C.F. 2008. White spot disease. European Community Reference Laboratory for Crustacean Diseases leaflet 2008.Google Scholar
- Pazir, M.K., Afsharnasab, M., Niamaymandi, N., Khaden, H., Akbarpour, E., and Zendebudi, A.A. 2012. Histopathological observation of white spot syndrome virus and infectious hypodermal and hematopoiectic necrosis virus in shrimp farms, Litopenaeus vannamei, in Bushehr Province, Iran. Asian J. Anim. Sci.6, 209–219.CrossRefGoogle Scholar
- Tsai, M.F., Yu, H.T., Tzeng, H.F., Leu, J.H., Chou, C.M., Huang, C.J., Wang, C.H., Lin, J.Y., Kou, G.H., and Lo, C.F. 2000. Identification and characterization of a shrimp white spot syndrome virus (WSSV) gene that encodes a novel chimeric polypeptide of cellular-type thymidine kinase and thymidylate kinase. Virology277, 100–110.PubMedCrossRefGoogle Scholar