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Journal of Microbiology

, Volume 51, Issue 4, pp 448–452 | Cite as

Phenotypic and genotypic analysis of clarithromycin-resistant Helicobacter pylori from Bogotá D.C., Colombia

  • Alba A. TrespalaciosEmail author
  • William Otero
  • Jorge E. Caminos
  • Marcela M. Mercado
  • Jenny Ávila
  • Liliana E. Rosero
  • Azucena Arévalo
  • Raúl A. Poutou-Piñales
  • David Y. Graham
Microbial Genetics, Genomics and Molecular Biology

Abstract

Resistance of Helicobacter pylori to clarithromycin is the most common cause of treatment failure in patients with H. pylori infections. This study describes the MICs and the presence of 23S rRNA mutations of H. pylori isolates from Bogotá, D.C., Colombia. H. pylori were isolated from gastric biopsies from patients with functional dyspepsia. Clarithromycin susceptibility was investigated by agar dilution and strains were considered resistant if the MIC was ≥1 μg/ml. DNA sequences of the 23S rRNA gene of strains resistant and sensitive to clarithromycin were determined to identify specific point mutations. Clarithromycin resistance was present in 13.6% of patients by agar dilution. The A2143G, A2142G and A2142C mutations were found in 90.5, 7.1, and 2.4% of H. pylori strains with resistance genotype.The resistant phenotype was associated with 23S rRNA resistance genotype in 85.7% of isolates. The point mutations in 23S rRNA were well correlated with MICs values for clarithromycin.

Keywords

Helicobacter pylori clarithromycin resistance 23S rRNA mutations 

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Copyright information

© The Microbiological Society of Korea and Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Alba A. Trespalacios
    • 1
    Email author
  • William Otero
    • 2
    • 3
  • Jorge E. Caminos
    • 4
  • Marcela M. Mercado
    • 1
  • Jenny Ávila
    • 1
  • Liliana E. Rosero
    • 1
  • Azucena Arévalo
    • 1
  • Raúl A. Poutou-Piñales
    • 5
  • David Y. Graham
    • 6
    • 7
  1. 1.Infectious Diseases Group, Microbiology Department, Science FacultyPontificia Universidad JaverianaBogotá, D.C.Colombia
  2. 2.Gastroenterology Unit. Clínica FundadoresBogotá, D.C.Colombia
  3. 3.Universidad Nacional de ColombiaBogotá, D.C.Colombia
  4. 4.Biochemistry UnitUniversidad Nacional de ColombiaBogotá, D.C.Colombia
  5. 5.Molecular Biotechnology Laboratory, Environment and Industrial Biotechnology Group (GBAI), Microbiology Department, Science FacultyPontificia Universidad JaverianaBogotá, D.C.Colombia
  6. 6.Department of MedicineMichael E. DeBakey Veterans Affairs Medical CenterHoustonUSA
  7. 7.Baylor College of MedicineHoustonUSA

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