Journal of Microbiology

, Volume 51, Issue 1, pp 130–135

Sublingual administration of bacteria-expressed influenza virus hemagglutinin 1 (HA1) induces protection against infection with 2009 pandemic H1N1 influenza virus

  • Byoung-Shik Shim
  • Jung-ah Choi
  • Ho-Hyun Song
  • Sung-Moo Park
  • In Su Cheon
  • Ji-Eun Jang
  • Sun Je Woo
  • Chung Hwan Cho
  • Min-Suk Song
  • Hyemi Kim
  • Kyung Joo Song
  • Jae Myun Lee
  • Suhng Wook Kim
  • Dae Sub Song
  • Young Ki Choi
  • Jae-Ouk Kim
  • Huan Huu Nguyen
  • Dong Wook Kim
  • Young Yil Bahk
  • Cheol-Heui Yun
  • Man Ki Song
Virology

DOI: 10.1007/s12275-013-2399-z

Cite this article as:
Shim, BS., Choi, J., Song, HH. et al. J Microbiol. (2013) 51: 130. doi:10.1007/s12275-013-2399-z

Abstract

Influenza viruses are respiratory pathogens that continue to pose a significantly high risk of morbidity and mortality of humans worldwide. Vaccination is one of the most effective strategies for minimizing damages by influenza outbreaks. In addition, rapid development and production of efficient vaccine with convenient administration is required in case of influenza pandemic. In this study, we generated recombinant influenza virus hemagglutinin protein 1 (sHA1) of 2009 pandemic influenza virus as a vaccine candidate using a well-established bacterial expression system and administered it into mice via sublingual (s.l.) route. We found that s.l. immunization with the recombinant sHA1 plus cholera toxin (CT) induced mucosal antibodies as well as systemic antibodies including neutralizing Abs and provided complete protection against infection with pandemic influenza virus A/CA/04/09 (H1N1) in mice. Indeed, the protection efficacy was comparable with that induced by intramuscular (i.m.) immunization route utilized as general administration route of influenza vaccine. These results suggest that s.l. vaccination with the recombinant non-glycosylated HA1 protein offers an alternative strategy to control influenza outbreaks including pandemics.

Keywords

Hemagglutinin mucosal immune response non-glycosylation pandemic sublingual 

Abbreviations

Abs

antibodies

ASCs

Ab secreting cells

BAL

Bronchoalveolar lavage

CLN

cervical lymph node

CNS

central nervous system

dpi

days post infection

EID

egg infective dose

HA

hemagglutinin

HI

Hemagglutination inhibition

i.n.

intranasal

i.m.

intramuscular

nAbs

neutralizing antibodies

SIgA

secretory IgA

sHA1

swine HA1

s.l.

sublingual

SN

Serum neutralization

Copyright information

© The Microbiological Society of Korea and Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Byoung-Shik Shim
    • 1
  • Jung-ah Choi
    • 1
  • Ho-Hyun Song
    • 1
  • Sung-Moo Park
    • 1
    • 2
  • In Su Cheon
    • 1
    • 2
  • Ji-Eun Jang
    • 1
  • Sun Je Woo
    • 1
    • 3
  • Chung Hwan Cho
    • 1
    • 2
  • Min-Suk Song
    • 4
  • Hyemi Kim
    • 5
  • Kyung Joo Song
    • 5
  • Jae Myun Lee
    • 5
  • Suhng Wook Kim
    • 6
  • Dae Sub Song
    • 7
  • Young Ki Choi
    • 4
  • Jae-Ouk Kim
    • 1
  • Huan Huu Nguyen
    • 1
  • Dong Wook Kim
    • 8
  • Young Yil Bahk
    • 9
  • Cheol-Heui Yun
    • 2
  • Man Ki Song
    • 1
  1. 1.Laboratory Science DivisionInternational Vaccine InstituteSeoulRepublic of Korea
  2. 2.Department of Agricultural Biotechnology and Research Institute for Agriculture and Life Sciences, and the Center for Agricultural Biomaterials, and Center for Food Safety and ToxicologySeoul National UniversitySeoulRepublic of Korea
  3. 3.Department of Oral Microbiology and Immunology, Dental Research Institute, and BK21 Program, School of DentistrySeoul National UniversitySeoulRepublic of Korea
  4. 4.College of Medicine and Medical Research InstituteChungbuk National UniversityCheongjuRepublic of Korea
  5. 5.Brain Korea 21 Project for Medical SciencesYonsei University College of MedicineSeoulRepublic of Korea
  6. 6.Department of Biomedical Science, College of Health SciencesKorea UniversitySeoulRepublic of Korea
  7. 7.Viral Infectious Disease Research CenterKorea Research Institute of Bioscience and BiotechnologyDaejeonRepublic of Korea
  8. 8.Department of Pharmacy, College of PharmacyHanyang UniversityKyeonggi-doRepublic of Korea
  9. 9.Department of BiotechnologyKonkuk UniversityChungjuRepublic of Korea

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