The Journal of Microbiology

, Volume 50, Issue 2, pp 359–362 | Cite as

GFP-expressing influenza a virus for evaluation of the efficacy of antiviral agents

  • Jin Il Kim
  • Sehee Park
  • Ilseob Lee
  • Sangmoo Lee
  • Saem Shin
  • Yongkwan Won
  • Min-Woong Hwang
  • Joon-Yong Bae
  • Jun Heo
  • Hye-Eun Hyun
  • Hyejin Jun
  • Soon Sung Lim
  • Man-Seong Park
Note

Abstract

To address its value as a screening tool in the development of antiviral drugs, a recombinant influenza virus expressing green fluorescent protein (rPR8-GFP virus) was investigated in vitro and in vivo. The inhibition of viral growth by a neuraminidase inhibitor in the cells or lower respiratory tracts of mice could be visualized by the level of fluorescence. In addition, the rPR8-GFP virus exhibited high pathogenicity in mice. Taken together, these results suggest that the rPR8-GFP virus can be a useful tool for the rapid identification of antiviral drugs active against influenza viruses.

Keywords

influenza green fluorescent protein antiviral drug 

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Copyright information

© The Microbiological Society of Korea and Springer-Verlag Berlin Heidelberg 2012

Authors and Affiliations

  • Jin Il Kim
    • 1
    • 2
  • Sehee Park
    • 1
    • 2
  • Ilseob Lee
    • 1
    • 2
  • Sangmoo Lee
    • 1
    • 2
  • Saem Shin
    • 1
    • 2
  • Yongkwan Won
    • 1
    • 2
  • Min-Woong Hwang
    • 1
    • 2
  • Joon-Yong Bae
    • 1
    • 2
  • Jun Heo
    • 1
    • 2
  • Hye-Eun Hyun
    • 1
    • 2
  • Hyejin Jun
    • 1
    • 2
  • Soon Sung Lim
    • 3
  • Man-Seong Park
    • 1
    • 2
  1. 1.Department of MicrobiologyHallym UniversityChuncheonRepublic of Korea
  2. 2.Center for Medical Science Research, College of MedicineHallym UniversityChuncheonRepublic of Korea
  3. 3.Department of Food Science and Nutrition, College of Natural SciencesHallym UniversityChuncheonRepublic of Korea

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