The Journal of Microbiology

, Volume 50, Issue 1, pp 50–57 | Cite as

Diversity and physiological properties of root endophytic actinobacteria in native herbaceous plants of Korea

  • Tae-Ui Kim
  • Sung-Heun Cho
  • Ji-Hye Han
  • Young Min Shin
  • Hyang Burm Lee
  • Seung Bum Kim
Articles

Abstract

Endophytic actinobacterial diversity in the native herbaceous plant species of Korea was analyzed using a culture-based approach. Sixty one actinobacterial strains were isolated, and assigned to 15 genera based on 16S rRNA gene analysis. The members of the genus Streptomyces comprised 45.9% of the total isolates, followed by Micromonospora (18.8%), Rhodococcus (6.6%), Microbispora (4.9%), and Micrococcus (4.9%). Other minor constituents included members of Microbacterium, Streptacidiphilus, Arthrobacter, Dietzia, Kitasatospora, Herbiconiux, Mycobacterium, Nocardia, Rathayibacter, and Tsukamurella. Among the isolates, 65.6% exhibited at least one hydrolytic enzyme activity out of four, and 45.9% exhibited antagonistic activity against at least one fungal pathogen out of five, thus demonstrating that endophytic actinobacteria can be an important source of bioactive compounds. Notably, most strains of Streptomyces proved active for both enzymatic and antagonistic activities.

Keywords

endophytic actinobacteria native herbaceous plant Streptomyces Rhodococcus 

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Copyright information

© The Microbiological Society of Korea and Springer-Verlag Berlin Heidelberg 2012

Authors and Affiliations

  • Tae-Ui Kim
    • 1
  • Sung-Heun Cho
    • 1
  • Ji-Hye Han
    • 1
  • Young Min Shin
    • 1
  • Hyang Burm Lee
    • 2
  • Seung Bum Kim
    • 1
  1. 1.Department of Microbiology and Molecular Biology, School of Bioscience and BiotechnologyChungnam National UniversityDaejeonRepublic of Korea
  2. 2.Environmental Microbiology Lab, Division of Applied Bioscience and BiotechnologyChonnam National UniversityGwangjuRepublic of Korea

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