Flavobacterium cheonhonense sp. nov., isolated from a freshwater reservoir
- 188 Downloads
A novel bacterium, designated strain ARSA-15T, was isolated from a freshwater sample collected from the Cheonho reservoir, Cheonan, Republic of Korea. The isolate was deep-yellow pigment, Gram-negative, rod-shaped, non-motile, and catalase- and oxidase-positive. Phylogenetic analysis based on 16S rRNA gene sequences showed that the isolate belongs to the genus Flavobacterium, and shared less than 97% sequence similarity with recognized Flavobacterium species. The novel species was able to grow at 10–37°C, pH 6.5–10.0, and in 0–0.5% (w/v) NaCl concentrations. Chemotaxonomically, iso-C15:1, iso-C15:0, and iso-C16:0 were observed to be the predominant cellular fatty acid, and menaquinone-6 (MK-6) was the predominant respiratory quinone. The major polar lipid patterns of strain ARSA-19T was phosphatidylethanolamine, unknown aminolipid (AL1 and AL2), and unidentified polar lipids (L1, L2, and L3). The genomic DNA G+C content of the isolate was 39.2 mol%. On the basis of polyphasic approach, strain ARSA-15T represents a novel species of the genus Flavobacterium, for which the name Flavobacterium cheonhonense sp. nov. is proposed. The type strain is ARSA-15T (=KACC 14967T =KCTC 23180T =JCM 17064T).
Keywordsfreshwater phylogenetic analysis Flavobacterium
Unable to display preview. Download preview PDF.
- Bergey, D.H., Harrison, F.C., Breed, R.S., Hammer, B.W., and Huntoon, F.M. 1923. Manual of Determinative Bacteriology. Williams and Wilkins, Baltimore, USA.Google Scholar
- Bernardet, J.F. and Bowman, J.P. 2011. Genus I. Flavobacterium Bergey et al. 1923. In Whitman, W. (ed.), Bergey’s Manual of Systematic Bacteriology, pp. 112–154, 2nd ed., Vol. 4, The Williams & Wilkins Co., Baltimore, USA.Google Scholar
- Bernardet, J.F., Segers, P., Vancanneyt, M., Berthe, F., Kersters, K., and Vandamme, P. 1996. Cutting a Gordian knot: emended classification and description of the genus Flavobacterium, emended description of the Family Flavobacteriaceae, and proposal of Flavobacterium hydatis nom. nov. (Basonym, Cytophaga aquatilis Strohl and Tait 1978). Int. J. Syst. Evol. Microbiol. 46, 128–148.Google Scholar
- Hall, T.A. 1999. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp. Ser.41, 95–98.Google Scholar
- Lane, D.J. 1991. 16S/23S rRNA sequencing. Nucleic acid techniques in bacterial systematics, pp. 115–175. In Stackebrandt, E. and Goodfellow, M. (eds.). Wiley, Chichester, UK.Google Scholar
- Moore, D.D. and Dowhan, D. 1995. Preparation and analysis of DNA. Current Protocols in Molecular Biology, pp. 2–11. In Ausubel, F.W., Brent, R., Kingston, R.E., Moore, D.D., Seidman, J.G., Smith, J.A., and Struhl, K. (eds.). Wiley, New York, N.Y., USA.Google Scholar
- Skerman, V.B.D. 1967. A guide to the identification of the Genera of Bacteria, 2nd edn. Williams & Wilkins, Baltimore, USA.Google Scholar
- Smibert, R.M. and Krieg, N.R. 1994. Phenotypic characterization. Methods for General and Molecular Bacteriology, pp. 607–654. In Gerhardt, P., Murray, R.G.E., Wood, W.A., and Krieg, N.R. (eds.). American Society for Microbiology, Washington, D.C., USA.Google Scholar