Journal of Microbiology

, Volume 50, Issue 4, pp 562–566 | Cite as

Flavobacterium cheonhonense sp. nov., isolated from a freshwater reservoir

  • Siwon Lee
  • Jung-Hwan Oh
  • Hang-Yeon Weon
  • Tae-Young Ahn


A novel bacterium, designated strain ARSA-15T, was isolated from a freshwater sample collected from the Cheonho reservoir, Cheonan, Republic of Korea. The isolate was deep-yellow pigment, Gram-negative, rod-shaped, non-motile, and catalase- and oxidase-positive. Phylogenetic analysis based on 16S rRNA gene sequences showed that the isolate belongs to the genus Flavobacterium, and shared less than 97% sequence similarity with recognized Flavobacterium species. The novel species was able to grow at 10–37°C, pH 6.5–10.0, and in 0–0.5% (w/v) NaCl concentrations. Chemotaxonomically, iso-C15:1, iso-C15:0, and iso-C16:0 were observed to be the predominant cellular fatty acid, and menaquinone-6 (MK-6) was the predominant respiratory quinone. The major polar lipid patterns of strain ARSA-19T was phosphatidylethanolamine, unknown aminolipid (AL1 and AL2), and unidentified polar lipids (L1, L2, and L3). The genomic DNA G+C content of the isolate was 39.2 mol%. On the basis of polyphasic approach, strain ARSA-15T represents a novel species of the genus Flavobacterium, for which the name Flavobacterium cheonhonense sp. nov. is proposed. The type strain is ARSA-15T (=KACC 14967T =KCTC 23180T =JCM 17064T).


freshwater phylogenetic analysis Flavobacterium 


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Copyright information

© The Microbiological Society of Korea and Springer-Verlag Berlin Heidelberg 2012

Authors and Affiliations

  • Siwon Lee
    • 1
  • Jung-Hwan Oh
    • 2
  • Hang-Yeon Weon
    • 3
  • Tae-Young Ahn
    • 1
  1. 1.Department of MicrobiologyDankook UniversityCheonanRepublic of Korea
  2. 2.Environmental Analysis & Research Team, The Environment Technology InstituteWoongjin Coway CO., Ltd.SeoulRepublic of Korea
  3. 3.Applied Microbiology Division, National Institute of Agricultural Science and TechnologyRural Development AdministrationSuwonRepublic of Korea

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