Bacillus kyonggiensis sp. nov., isolated from soil of a lettuce field
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A Gram-positive, rod-shaped, motile, endospore-forming bacterial strain, designated NB22T, was isolated from soil of a lettuce field in Kyonggi province, South Korea, and was characterized by using a polyphasic taxonomic approach. This novel isolate grew optimally at 30–37°C and pH 8–9. It grew in the presence of 0–4% NaCl (optimum, 1–2%). Comparative 16S rRNA gene sequence analysis showed that strain NB22T was closely related to members of the genus Bacillus and fell within a coherent cluster comprising B. siralis 171544T (98.1%) and B. korlensis ZLC-26T (97.3%). The levels of 16S rRNA gene sequence similarity with respect to other Bacillus species with validly published names were less than 96.4%. Strain NB22T had a genomic DNA G+C content of 36.3 mol% and the predominant respiratory quinone was MK-7. The peptidoglycan contained meso-diaminopimelic acid. The major cellular fatty acids were iso-C15:0, anteiso-C15:0, C14:0, and C16:0. These chemotaxonomic results supported the affiliation of strain NB22T to the genus Bacillus, and the low DNA-DNA relatedness values and distinguishing phenotypic characteristics allowed genotypic and phenotypic differentiation of strain NB22T from recognized Bacillus species. On the basis of the evidence presented, strain NB22T is considered to represent a novel species of the genus Bacillus, for which the name Bacillus kyonggiensis sp. nov. is proposed. The type strain is NB22T (=KEMB 5401-267T =JCM 17569T).
Keywordstaxonomy 16S rRNA gene Bacillaceae Bacillus kyonggiensis
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- Baron, S. 1996. Chapter 15, Bacillus. In Medical Microbiology 4th edition. University of Texas Medical Branch at Galveston, Galveston, USA.Google Scholar
- Claus, D. and R.C.W. Berkeley. 1986. Genus Bacillus Cohn 1872. In Bergey’s Manual of Systematic Bacteriology, vol. 2, pp. 1105–1140. Williams and Wilkins, Baltimore, USA.Google Scholar
- Doetsch, R.N. 1981. Determinative methods of light microscopy. In Manual of Methods for General Bacteriology, pp. 21–33. American Society for Microbiology, Washington, D.C., USA.Google Scholar
- Euzéby, J.P. 2008. List of Prokaryotic Names with Standing in Nomenclature. http://www.bacterio.cict.fr/
- Ezaki, T., Y. Hashimoto, and E. Yabuuchi. 1989. Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int. J. Syst. Bacteriol. 39, 224–229.CrossRefGoogle Scholar
- Hall, T.A. 1999. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp. Ser. 41, 95–98.Google Scholar
- Kimura, M. 1983. The Neutral Theory of Molecular Evolution. Cambridge University Press. Cambridge, UK.Google Scholar
- Sasser, M. 1990. Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids, MIDI Technical Note 101. MIDI Inc., Newark, DE, USA.Google Scholar
- Shida, O., H. Takagi, K. Kadowaki, L.K. Nakamura, and K. Komagata. 1997. Transfer of Bacillus alginolyticus, Bacillus chondroitinus, Bacillus curdlanolyticus, Bacillus glucanolyticus, Bacillus kobensis, and Bacillus thiaminolyticus to the genus Paenibacillus and emended description of the genus. Int. J. Syst. Bacteriol. 47, 289–298.PubMedCrossRefGoogle Scholar
- Wayne, L.G., D.J. Brenner, R.R. Colwell, P.A.D. Grimont, O. Kandler, M.I. Krichevsky, L.H. Moore, and et al. 1987. International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int. J. Syst. Bacteriol. 37, 463–464.Google Scholar
- Wilson, K. 1987. Preparation of genomic DNA from bacteria. In Current Protocols in Molecular Biology, pp. 2.4.1–2.4.5. Green Publishing and Wiley-Interscience, New York, NY, USA.Google Scholar