The Journal of Microbiology

, Volume 49, Issue 6, pp 1027–1032 | Cite as

Bacillus manliponensis sp. nov., a new member of the Bacillus cereus group isolated from foreshore tidal flat sediment

  • Min Young Jung
  • Joong-Su Kim
  • Woon Kee Paek
  • Jeongheui Lim
  • Hansoo Lee
  • Pyoung Il Kim
  • Jin Yeul Ma
  • Wonyong Kim
  • Young-Hyo Chang
Note

Abstract

A Gram-positive, endospore-forming, new Bacillus species, strain BL4-6T, was isolated from tidal flat sediment of the Yellow Sea. Strain BL4-6T is a straight rod, with motility by peritrichate flagella. The cell wall contains meso-diaminopimelic acid, and the major respiratory quinone is menaquinone-7. The major fatty acids are iso-C15:0 and summed feature 3 (containing C16:1 ω7c/iso-C15:0 2OH, and/or iso-C15:0 2OH/C16:1 ω7c). Cells are catalase-positive and oxidase-negative. The G+C content of the genomic DNA is 38.0 mol%. Based on a comparative 16S rRNA gene sequence analysis, the isolate belongs to the genus Bacillus, forms a clade with the Bacillus cereus group, and is closely related to Bacillus mycoides (98.5%), Bacillus cereus (98.5%), Bacillus anthracis (98.4%), Bacillus thuringiensis (98.4%), Bacillus weihenstephanensis (98.1%), and Bacillus pseudomycoides (97.5%). The isolate showed less than 85% similarity of the gyrA gene sequence and below 95% similarity of the rpoB gene sequence to the members of this group. DNA-DNA relatedness between strain BL4-6T and B. cereus group was found to be in a range of 22.8–42.3%, and thus BL4-6T represents a unique species. On the basis of these studies, strain BL4-6T (=KCTC 13319T =JCM 15802T) is proposed to represent the type strain of a novel species, Bacillus manliponensis sp. nov.

Keywords

Bacillus cereus group Bacillus manliponensis sp. nov. phylogenetic new species 

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Copyright information

© The Microbiological Society of Korea and Springer-Verlag Berlin Heidelberg  2011

Authors and Affiliations

  • Min Young Jung
    • 1
    • 7
  • Joong-Su Kim
    • 2
  • Woon Kee Paek
    • 3
  • Jeongheui Lim
    • 1
  • Hansoo Lee
    • 4
  • Pyoung Il Kim
    • 5
  • Jin Yeul Ma
    • 6
  • Wonyong Kim
    • 7
  • Young-Hyo Chang
    • 1
  1. 1.Korean Collection for Type Cultures, Biological Resource CenterKRIBBDaejeonRepublic of Korea
  2. 2.Molecular Bioprocess Research Center, Jeonbuk Branch InstituteKRIBBJeonbukRepublic of Korea
  3. 3.National Science MuseumDaejeonRepublic of Korea
  4. 4.Korea Institute of Environmental EcologyDaejeonRepublic of Korea
  5. 5.School of Chemical and Biological Engineering, Institute of Molecular Biology and GeneticsSeoul National UniversitySeoulRepublic of Korea
  6. 6.Center for Herbal Medicine Improvement ResearchKorea Institute of Oriental MedicineDaejeonRepublic of Korea
  7. 7.Department of Microbiology, College of MedicineChung-Ang UniversitySeoulRepublic of Korea

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