Aquimarina litoralis sp. nov., isolated from a coastal seawater
- 102 Downloads
A strictly aerobic, red-pigmented, non-motile, catalase- and oxidase-positive, Gram-staining-negative bacterium, designated strain CNURIC011T, was isolated from seawater off the coast of Jeju Island in Korea. A phylogenetic analysis based on 16S rRNA gene sequences showed that strain CNURIC011T belongs to the genus Aquimarina in the family Flavobacteriaceae. 16S rRNA gene sequence analysis revealed that the close relatives of the novel strain are Aquimarina latercula ATCC 23177T, Aquimarina marcrocephali JAMB N27T, Aquimarina intermedia KMM 6258T, Aquimarina muelleri KMM 6020T, and Aquimarina brevivitae SMK-19T, with sequence similarities of 97.6, 96.6, 96.0, 95.6, and 94.2%, respectively. DNA-DNA hybridization revealed that the level of relatedness between strain CNURIC011T and Aquimarina latercula ATCC 23177T (=KCTC 2912T) was 4.9%. The DNA G+C content was 35.8 mol% and the major respiratory quinone was MK-6. The major fatty acids were iso-C15:0 (14.9%), C15:0 (13.9%), iso-C17:0 3-OH (12.6%), iso-C15:1 G (7.3%), and iso-C17:1 ω9c (7.2%). On the basis of phenotypic, phylogenetic, and genotypic data, strain CNURIC011T represents a novel species within the genus Aquimarina, for which the name Aquimarina litoralis sp. nov. is proposed. The type strain is CNURIC011T (=KCTC 22614T =JCM 15974T).
KeywordsAquimarina litoralis sp. nov. Flavobacteriaceae taxonomy seawater
Unable to display preview. Download preview PDF.
- Brown, T. 2005. Dot and slot blotting of DNA onto a positively charged nylon membrane using a manifold. Current Protocols in Molecular Biology, pp. 2.9.18–2.9.20. In F.M. Ausubel, R. Brent, R.E. Kingston, D.D. Moore, J.G. Seidman, J.A. Smith, and K. Struhl (eds.). Wiley, New York, N.Y., USA.Google Scholar
- Cole, J.R., B. Chai, T.L. Marsh, R.J. Farris, Q. Wang, S.A. Kulam, S. Chandra, D.M. McGarrell, T.M. Schmidt, G.M. Garrity, and J.M. Tiedje. 2003. The Ribosomal Database Project (RDP-II): previewing a new autoaligner that allows regular updates and the new prokaryotic taxonomy. Nucleic Acids Res. 31, 442–443.CrossRefPubMedGoogle Scholar
- Hall, T.A. 1999. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp. Ser. 41, 95–98.Google Scholar
- Lane, D.J. 1991. 16S/23S rRNA sequencing. Nucleic Acid Techniques in Bacterial Systematics, pp. 115–175. In E. Stackebrandt and M. Goodfellow (eds.). Wiley, New York, N.Y., USA.Google Scholar
- McCammon, S.A. and J.P. Bowman. 2000. Taxonomy of Antarctic Flavobacterium species: description of Flavobacterium gillisiae sp. nov., Flavobacterium tegetincola sp. nov. and Flavobacterium xanthum sp. nov., nom. rev. and reclassification of [Flavobacterium] salegens as Salegentibacter salegens gen. nov., comb. nov. Int. J. Syst. Evol. Microbiol. 50, 1055–1063.PubMedGoogle Scholar
- Miyazaki, M., Y. Nagano, Y. Fujiwara, Y. Hatada, and Y. Nogi. 2009. Aquimarina marcrocephali sp. nov., isolated from the sediment agjacent to sperm whale carcasses off Kagoshima, Japan. Int. J. Syst. Evol. Microbiol. Article in press.Google Scholar
- Nedashkovskaya, O.I., S.B. Kim, A.M. Lysenko, G.M. Frolova, V.V. Mikhailov, K.H. Lee, and K.S. Bae. 2005. Description of Aquimarina muelleri gen. nov., sp. nov., and proposal of the reclassification of [Cytophaga] latercula Lewin 1969 as Stanierella latercula gen. nov., comb. nov. Int. J. Syst. Evol. Microbiol. 55, 225–229.CrossRefPubMedGoogle Scholar
- Nedashkovskaya, O.I., M. Vancanneyt, L. Christiaens, N.I. Kalinovskaya, V.V. Mikhailov, and J. Swings. 2006. Aquimarina intermedia sp. nov., reclassification of Stanierella latercula (Lewin 1969) as Aquimarina latercula comb. nov. and Gaetbulimicrobium brevivitae Yoon et al. 2006 as Aquimarina brevivitae comb. nov. and emended description of the genus Aquimarina. Int. J. Syst. Evol. Microbiol. 56, 2037–2041.CrossRefPubMedGoogle Scholar
- Tindall, B.J., J. Sikorski, R.A. Simbert, and N.R. Kreig. 2007. Phenotypic characterization and principles of comparative systematics. Methods for General and Molecular Microbiology, pp. 330–393. In C.A. Reddy, T.J. Beveridge, J.A. Breznak, G.A. Marzluf, T.M. Schmidt, and L.R. Snyder (eds.). American Society for Microbiology, Washington, D.C., USA.Google Scholar
- Wayne, L.G., D.J. Brenner, R.R. Colwell, P.A.D. Grimont, O. Kandler, M.I. Krichevsky, L.H. Moore, and et al. 1987. International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int. J. Syst. Bacteriol. 37, 463–464.CrossRefGoogle Scholar
- ZoBell, C.E. 1941. Studies on marine bacteria. I. The cultural requirements of heterotrophic aerobes. J. Mar. Res. 4, 42–75.Google Scholar