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The Journal of Microbiology

, Volume 48, Issue 2, pp 223–228 | Cite as

Protection against Helicobacter pylori infection by a trivalent fusion vaccine based on a fragment of urease B-UreB414

  • Li Wang
  • Xiao-Fei Liu
  • Shi Yun
  • Xiao-Peng Yuan
  • Xu-Hu Mao
  • Chao Wu
  • Wei-Jun Zhang
  • Kai-Yun Liu
  • Gang Guo
  • Dong-Shui Lu
  • Wen-De Tong
  • Ai-Dong Wen
  • Quan-Ming Zou
Article

Abstract

A multivalent fusion vaccine is a promising option for protection against Helicobacter pylori infection. In this study, UreB414 was identified as an antigenic fragment of urease B subunit (UreB) and it induced an antibody inhibiting urease activity. Immunization with UreB414 partially protected mice from H. pylori infection. Furthermore, a trivalent fusion vaccine was constructed by genetically linking heat shock protein A (HspA), H. pylori adhesin A (HpaA), and UreB414, resulting in recombinant HspA-HpaA-UreB414 (rHHU). Its protective effect against H. pylori infection was tested in BALB/c mice. Oral administration of rHHU significantly protected mice from H. pylori infection, which was associated with H. pylori-specific antibody production and Th1/Th2-type immune responses. The results show that a trivalent fusion vaccine efficiently combats H. pylori infection, and that an antigenic fragment of the protein can be used instead of the whole protein to construct a multivalent vaccine.

Keywords

H. pylori multivalent vaccine urease B subunit H. pylori adhesin A heat shock protein A 

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Copyright information

© The Microbiological Society of Korea and Springer-Verlag Berlin Heidelberg 2010

Authors and Affiliations

  • Li Wang
    • 1
  • Xiao-Fei Liu
    • 2
  • Shi Yun
    • 2
  • Xiao-Peng Yuan
    • 2
    • 3
  • Xu-Hu Mao
    • 2
  • Chao Wu
    • 2
  • Wei-Jun Zhang
    • 2
  • Kai-Yun Liu
    • 2
  • Gang Guo
    • 2
  • Dong-Shui Lu
    • 2
  • Wen-De Tong
    • 2
  • Ai-Dong Wen
    • 1
  • Quan-Ming Zou
    • 2
  1. 1.Department of Pharmacy, Xijing Hospitalthe Fourth Military Medical UniversityXi’anP. R. China
  2. 2.Department of Clinical Microbiology and Immunology, School of Medical Laboratorythe Third Military Medical UniversityChongqingP. R. China
  3. 3.Department of Clinical Laboratory, Zhujiang HospitalSouthern Medical UniversityGuangzhouP. R. China

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