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The Journal of Microbiology

, Volume 47, Issue 3, pp 337–343 | Cite as

Nup211, the fission yeast homolog of Mlp1/Tpr, is involved in mRNA export

  • Jin-Ah Bae
  • DongGeRaMi Moon
  • Jin Ho YoonEmail author
Article

Abstract

Synthetic lethal mutants have been previously isolated in fission yeast Schizosaccharomyces pombe, which genetically interact with spmex67, in order to identify the genes involved in mRNA export. The nup211 gene was isolated by complementation of the growth defect in one of the synthetic lethal mutants, SLMex2, under synthetic lethal condition. We showed that Nup211, fission yeast homolog of Mlpl/Mlp2/Tpr, is essential for vegetative growth and Nup211-GFP proteins expressed at endogenous level are localized mainly in nuclear periphery. The accumulation of poly(A)+ RNA in the nucleus is exhibited when expression of nup211 is repressed or over-expressed. These results suggest that the Nup211 protein plays a pivotal role of mRNA export in fission yeast.

Keywords

mRNA export Schizosaccharomyces pombe nup211 

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Copyright information

© The Microbiological Society of Korea and Springer-Verlag Berlin Heidelber GmbH 2009

Authors and Affiliations

  1. 1.Department of EducationSungshin Women’s UniversitySeoulRepublic of Korea
  2. 2.School of Biological Sciences and Chemistry, and Basic Sciences Research InstituteSungshin Women’s UniversitySeoulRepublic of Korea

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