The Journal of Microbiology

, 47:651 | Cite as

Biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) copolyesters with a high molar fraction of 3-hydroxyvalerate by an insect-symbiotic Burkholderia sp. IS-01

  • Do Young Kim
  • Doo-Sang Park
  • Soon Bum Kwon
  • Moon Gyu Chung
  • Kyung Sook Bae
  • Ho-Yong Park
  • Young Ha Rhee
Note

Abstract

Burkholderia sp. IS-01 capable of biosynthesizing poly(3-hydroxybutyrate-co-3-hydroxyvalerate) [poly(3HB-co-3HV)] copolyesters with a high molar fraction of 3HV was isolated from the gut of the adult longicorn beetle, Moechotypa diphysis. The strain IS-01 was relatively tolerant to high concentrations of levulinic acid and accumulated a poly(13.5 mol% 3HB-co-86.5 mol% 3HV) copolyester when cultivated on a mixture of gluconate (20 g/L) and levulinic acid (12.5 g/L). In this case, the content of the copolyester in the cells was approximately 60.0%. The compositions of the copolyesters were easily regulated by altering the molar ratio of gluconate and levulinic acid in the medium. The organism was found to possess a class I PHA synthase (PhaC) gene (1,881 bp) that encodes a protein with a deduced molecular mass of 68,538 Da that consists of 626 amino acids. The PhaC of this organism was most similar to that of B. cenocepacia PC184 (92% similarity).

Keywords

Burkholderia insect-symbiotic microorganism levulinic acid polyhydroxyalkanoates (PHAs) poly(3-hydroxybutyrate-co-3-hydroxyvalerate) [poly(3HB-co-3HV)] PHA synthase 

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Copyright information

© The Microbiological Society of Korea and Springer Berlin Heidelberg 2009

Authors and Affiliations

  • Do Young Kim
    • 1
  • Doo-Sang Park
    • 2
  • Soon Bum Kwon
    • 3
  • Moon Gyu Chung
    • 3
  • Kyung Sook Bae
    • 2
  • Ho-Yong Park
    • 1
  • Young Ha Rhee
    • 3
  1. 1.Industrial Bio-materials Research CenterKRIBBDaejeonRepublic of Korea
  2. 2.Biological Resources CenterKRIBBDaejeonRepublic of Korea
  3. 3.Department of MicrobiologyChungnam National UniversityDaejeonRepublic of Korea

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