Halorubrum cibi sp. nov., an extremely halophilic archaeon from salt-fermented seafood
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Strain B31T is a Gram-staining-negative, motile, and extremely halophilic archaeon that was isolated from salt-fermented seafood. Its morphology, physiology, biochemical features, and 16S rRNA gene sequence were determined. Phylogenetic analysis of its 16S rRNA gene sequence and composition of its major polar lipids placed this archaeon in the genus Halorubrum of the family Halobacteriaceae. Strain B31T showed 97.3, 97.2, and 96.9 % 16S rRNA similarity to the type strains of Halorubrum alkaliphilum, Hrr. tibetense, and Hrr. vacuolatum, respectively. Its major polar lipids were phosphatidylglycerol (PG), phosphatidylglycerol phosphate methyl ester (PGP-Me) and sulfated diglycosyl diether (S-DGD). Genomic DNA from strain B31T has a 61.7 mol% G+C content. Analysis of 16S rRNA gene sequences, as well as physiological and biochemical tests, identified genotypic and phenotypic differences between strain B31T and other Halorubrum species. The type strain of the novel species is B31T (=JCM 15757T =DSM 19504T).
KeywordsHalorubrum cibi sp. nov. archaeon taxonomy salt-fermented seafood
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- Ezaki, T., H. Hashimoto, and E. Yabuuchi. 1989. Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int. J. Syst. Bacteriol. 39, 224–229.CrossRefGoogle Scholar
- Felsenstein, J. 2005. PHYLIP — Phylogeny Inference Package, version 3.6. Distributed by the author. University of Washington, Seattle, WA, USA.Google Scholar
- Gerhardt, P., R.G.E. Murray, W.A. Wood, and N.R. Krieg. 1994. Methods for general and molecular bacteriology. American Society for Microbiology, Washington, D.C., USA.Google Scholar
- McGenity, T.J. and W.D. Grant. 1995. Transfer of Halobacterium saccharovorum, Halobacterium sodomense, Halobacterium trapanicum NRC 34021 and Halobacterium lacusprofundi to the genus Halorubrum gen. nov., as Halorubrum saccharovorum comb. nov., Halorubrum sodomense comb. nov., Halorubrum trapanicum comb. nov., and Halorubrum lacusprofundi comb. nov. Syst. Appl. Microbiol. 18, 237–243.Google Scholar
- McGenity, T.J. and W.D. Grant. 2001. Genus VII. Halorubrum, p. 320–324. In D.R. Boone, R.W. Castenholz, and G.M. Garrity (eds.), Bergey’s Manual of Systematic Bacteriology, Springer, New York, N.Y., USA.Google Scholar
- Sambrook, J., E.F. Fritsch, and T. Maniatis. 1989. Molecular Cloning: a Laboratory Manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, N.Y., USA.Google Scholar
- Suh, H.K. and S.S. Yoon. 1987. A study on the regional characteristics of Korean chotkal. Korean J. Dietary Culture 2, 45–54.Google Scholar