The Journal of Microbiology

, Volume 47, Issue 3, pp 270–276 | Cite as

Effect of glycosylation on the biochemical properties of β-xylosidases from Aspergillus versicolor

  • Alexandre Favarin Somera
  • Marita Gimenez Pereira
  • Luis Henrique Souza Guimarães
  • Maria de Lourdes Teixeira de Moraes Polizeli
  • Héctor Francisco Terenzi
  • Rosa Prazeres Melo Furriel
  • João Atílio Jorge
Article
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Abstract

Aspergillus versicolor grown on xylan or xylose produces two β-xylosidases with differences in biochemical properties and degree of glycosylation. We investigated the alterations in the biochemical properties of these β-xylosidases after deglycosylation with Endo-H or PNGase F. After deglycosylation, both enzymes migrated faster in PAGE or SDS-PAGE exhibiting the same Rf. Temperature optimum of xylan-induced and xylose-induced β-xylosidases was 45°C and 40°C, respectively, and 35°C after deglycosylation. The xylan-induced enzyme was more active at acidic pH. After deglycosylation, both enzymes had the same pH optimum of 6.0. Thermal resistance at 55°C showed half-life of 15 min and 9 min for xylose- and xylan-induced enzymes, respectively. After deglycosylation, both enzymes exhibited half-lives of 7.5 min. Native enzymes exhibited different responses to ions, while deglycosylated enzymes exhibited identical responses. Limited proteolysis yielded similar polypeptide profiles for the deglycosylated enzymes, suggesting a common polypeptide core with differential glycosylation apparently responsible for their biochemical and biophysical differences.

Keywords

β-xylosidase xylobiase glycosylation deglycosylation glycoprotein Aspergillus versicolor 
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Copyright information

© The Microbiological Society of Korea and Springer-Verlag Berlin Heidelber GmbH 2009

Authors and Affiliations

  • Alexandre Favarin Somera
    • 1
  • Marita Gimenez Pereira
    • 1
  • Luis Henrique Souza Guimarães
    • 1
  • Maria de Lourdes Teixeira de Moraes Polizeli
    • 1
  • Héctor Francisco Terenzi
    • 1
  • Rosa Prazeres Melo Furriel
    • 2
  • João Atílio Jorge
    • 1
  1. 1.Departamentos de Biologia e QuímicaUniversidade de São PauloRibeirão PretoBrasil
  2. 2.Faculdade de Filosofia, Ciências e Letras de Ribeirão PretoUniversidade de São PauloRibeirão PretoBrasil

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