The Journal of Microbiology

, Volume 47, Issue 4, pp 371–384

Molecular analysis of prokaryotic diversity in the deep subsurface of the former Homestake gold mine, South Dakota, USA

  • Gurdeep Rastogi
  • Larry D. Stetler
  • Brent M. Peyton
  • Rajesh K. Sani
Articles

Abstract

A culture-independent molecular phylogenetic analysis was carried out to study the prokaryotic diversity in two soil samples collected from the subsurface (1.34 km depth) of the former Homestake gold mine, Lead, South Dakota, USA at two sites, the Ross shaft and number 6 Winze. Microbial community analyses were performed by cloning and sequencing of 16S rRNA genes retrieved directly from soil samples. Geochemical characterization of soils revealed high amount of toxic metals such as As, Cd, Co, Cr, Cu, Ni, Pb, Zn, and U at both the sites. Phylogenetic analyses showed that soil samples were predominantly composed of phylotypes related to phylum Proteobacteria. Other phyla detected in libraries were Acidobacteria, Actinobacteria, Bacteroidetes, Chloroflexi, Chlorobi, Firmicutes, Gemmatimonadetes, Nitrospirae, Planctomycetes, Verrucomicrobia, and candidate divisions OP10 and TM7. The majority (>95%) of the phylotypes retrieved in the libraries were most closely related to environmental sequences from yet-uncultured bacteria representing a hitherto unidentified diversity. The archaeal communities at both the sites exhibited lower diversity and were most closely affiliated to uncultivated species within the Crenarchaeota. Results showed the existence of diverse microbial populations in deep subsurface environment of the Homestake gold mine. Statistical analyses demonstrated that each site harbored phylogenetically distinct microbial populations that were more diverse at Ross site compare to winze site.

Keywords

Homestake DUSEL gold mine microbial diversity 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Copyright information

© The Microbiological Society of Korea and Springer Berlin Heidelberg 2009

Authors and Affiliations

  • Gurdeep Rastogi
    • 1
  • Larry D. Stetler
    • 2
  • Brent M. Peyton
    • 3
  • Rajesh K. Sani
    • 1
  1. 1.Department of Chemical and Biological EngineeringSouth Dakota School of Mines and TechnologyRapid CityUSA
  2. 2.Department of Geology and Geological EngineeringSouth Dakota School of Mines and TechnologyRapid CityUSA
  3. 3.Department of Chemical and Biological EngineeringMontana State UniversityBozemanUSA
  4. 4.Department of Plant PathologyUniversity of CaliforniaDavisUSA

Personalised recommendations