Protective effect of polygoni cuspidati radix and emodin on Vibrio vulnificus cytotoxicity and infection
- 181 Downloads
Vibrio vulnificus, a good model organism of bacterial septicemia, causes fatal septicemia manifesting a fulminating course and a high mortality rate within days. In order to identify new natural substances preventing V. vulnificus infection, a plant library was screened for inhibiting cytotoxicity to host cells by using Trypan blue staining and LDH assay. We found that Polygoni Cuspidati Radix potently suppressed the acute death of HeLa and RAW264.7 cells in a dose dependent manner. Further studies revealed that Polygoni Cuspidati Radix inhibited V. vulnificus growth and survival in HI broth and seawater, respectively. We confirmed that Polygoni Cuspidati Radix contained high level of emodin by thin layer chromatography (TLC). Emodin showed direct antibacterial activity against V. vulnificus. In addition, emodin prevented the morphologic damages and acute death of HeLa cells caused from V. vulnificus. The safety of Polygoni Cuspidati Radix and emodin to host cells was confirmed by MTT assay. Polygoni Cuspidati Radix and emodin protected mice from V. vulnificus infection.
KeywordsV. vulnificus infection emodin Polygoni Cuspidati Radix cytotoxicity antibacterial activity
Unable to display preview. Download preview PDF.
- Baker-Austin, C., J.V. McArthur, A.H. Lindell, M.S. Wright, R.C. Tuckfield, J. Gooch, L. Warner, J. Oliver, and R. Stepanauskas. 2008. Multi-site analysis reveals widespread antibiotic resistance in the marine pathogen Vibrio vulnificus. Microb. Ecol. In press.Google Scholar
- Kim, Y.R., S.E. Lee, C.M. Kim, S.Y. Kim, E.K. Shin, D.H. Shin, S.S. Chung, H.E. Choy, A. Progulske-Fox, J.D. Hillman, M. Handfield, and J.H. Rhee. 2003. Characterization and pathogenic significance of Vibrio vulnificus antigens preferentially expressed in septicemic patients. Infect. Immun. 71, 5461–5471.PubMedCrossRefGoogle Scholar
- Radu, S., N. Elhadi, Z. Hassan, G. Rusul, S. Lihan, N. Fifadara, Yuherman, and E. Purwati. 1998. Characterization of Vibrio vulnificus isolated from cockles (Anadara granosa): antimicrobial resistance, plasmid profiles and random amplification of polymorphic DNA analysis. FEMS Microbiol. Lett. 165, 139–143.PubMedCrossRefGoogle Scholar
- Shuangsuo, D., Z. Zhengguo, C. Yunru, Z. Xin, W. Baofeng, Y. Lichao, and C. Yan’an. 2006. Inhibition of the replication of hepatitis B virus in vitro by emodin. Med. Set Monit. 12, BR302–306.Google Scholar