The Journal of Microbiology

, Volume 47, Issue 1, pp 101–109

Antimicrobial activity of enterocins from Enterococcus faecalis SL-5 against Propionibacterium acnes, the causative agent in acne vulgaris, and its therapeutic effect

  • Bong Seon Kang
  • Jae-Gu Seo
  • Gwa-Su Lee
  • Jung-Hwa Kim
  • Sei Yeon Kim
  • Ye Won Han
  • Hoon Kang
  • Hyung Ok Kim
  • Ji Hwan Rhee
  • Myung-Jun Chung
  • Young Min Park
Article

Abstract

A lactic acid bacterial strain was isolated from human fecal specimen and identified as Enterococcus faecalis SL-5. The isolated strain showed antimicrobial activity against Gram-positive pathogens assayed, especially the highest activity against Propionibacterium acnes. The antimicrobial substance was purified and verified as a bacteriocin (named ESL5) of E. faecalis SL-5 by activity-staining using P. acnes as an indicator. N-terminal sequence of ESL5 was determined (MGAIAKLVAK) and sequence analysis revealed that it is almost identical to the some of enterocins including L50A/B of E. faecium L50 and MR10A/B of E. faecalis MRR 10-3. From the sequencing data of L50A/B structural genes, the nucleotide sequence showed 100% identity with that of the MR10A/B structural genes, implying that ESL5 is an equivalent of enterocin MR10. Meanwhile, we also tested the therapeutic effect of anti-P. acnes activity in patients with mild to moderate acne because of its pathogenic role to acne vulgaris. For this purpose, a concentrated powder of CBT SL-5 was prepared using cell-free culture supernatant (CFCS) of E. faecalis SL-5 and included in a lotion for application in the patients. The study showed that CBT SL-5 lotion significantly reduced the inflammatory lesions like pustules compared to the placebo lotion. Therefore our results indicate that the anti-P. acnes activity produced by E. faecalis SL-5 has potential role to the treatment of acne as an alternative to topical antibiotics.

Keywords

acne vulgaris antimicrobial activity cell-free culture supernatant 

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Copyright information

© The Microbiological Society of Korea and Springer-Verlag Berlin Heidelber GmbH 2009

Authors and Affiliations

  • Bong Seon Kang
    • 1
  • Jae-Gu Seo
    • 3
  • Gwa-Su Lee
    • 3
  • Jung-Hwa Kim
    • 3
  • Sei Yeon Kim
    • 1
  • Ye Won Han
    • 1
  • Hoon Kang
    • 2
  • Hyung Ok Kim
    • 1
  • Ji Hwan Rhee
    • 3
  • Myung-Jun Chung
    • 3
  • Young Min Park
    • 1
  1. 1.Department of DermatologyKangnam St. Mary’s HospitalSeoulRepublic of Korea
  2. 2.St. Paul’s Hospital, College of MedicineThe Catholic University of KoreaSeoulRepublic of Korea
  3. 3.R&D Center, Cellbiotech, Co. Ltd.GimpoRepublic of Korea

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