The Journal of Microbiology

, Volume 46, Issue 6, pp 704–708 | Cite as

Molecular and phylogenetic characterization of Spodoptera litura granulovirus

  • Yong Wang
  • Jae Young Choi
  • Jong Yul Roh
  • Soo Dong Woo
  • Byung Rae Jin
  • Yeon Ho Je
Article

Abstract

Baculovirus, Spodoptera litura granulovirus (SlGV) was isolated from the infected S. litura larvae, and was characterized. The granule of SlGV was ovoidal shape with an approximate size of 240∼340 nm× 140∼180 nm. Each granule contained one single rod-shape virion with a mean size of 180∼200 nm×20∼40 nm. Restriction endonuclease fragment analysis estimated that the total genome size of SlGV is about 115 kb. Necleotide sequence analysis of the granulin gene showed that the gene encodes 249 amino acids with a predicted molecular mass of 29 kDa. When the phylogenic relationship was analyzed using the nucleotide sequence of the granulin gene, SlGV was most closely related to Trichoplusia ni granulovirus (TnGV) and Xestia c-nigrum granulovirus (XcGV) which belong to Type I granulovirus.

Keywords

baculovirus Spodoptera litura granulovirus SlGV granulin 

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Copyright information

© The Microbiological Society of Korea and Springer-Verlag Berlin Heidelber GmbH 2008

Authors and Affiliations

  • Yong Wang
    • 1
  • Jae Young Choi
    • 2
  • Jong Yul Roh
    • 1
  • Soo Dong Woo
    • 3
  • Byung Rae Jin
    • 4
  • Yeon Ho Je
    • 1
  1. 1.Department of Agricultural Biotechnology, College of Agriculture and Life SciencesSeoul National UniversitySeoulRepublic of Korea
  2. 2.Research Institute for Agriculture and Life SciencesSeoul National UniversitySeoulRepublic of Korea
  3. 3.College of Agriculture, Life and Environment SciencesChungbuk National UniversityCheongjuRepublic of Korea
  4. 4.College of Natural Resources and Life ScienceDong-A UniversityPusanRepublic of Korea

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