Molecular and phylogenetic characterization of Spodoptera litura granulovirus
Baculovirus, Spodoptera litura granulovirus (SlGV) was isolated from the infected S. litura larvae, and was characterized. The granule of SlGV was ovoidal shape with an approximate size of 240∼340 nm× 140∼180 nm. Each granule contained one single rod-shape virion with a mean size of 180∼200 nm×20∼40 nm. Restriction endonuclease fragment analysis estimated that the total genome size of SlGV is about 115 kb. Necleotide sequence analysis of the granulin gene showed that the gene encodes 249 amino acids with a predicted molecular mass of 29 kDa. When the phylogenic relationship was analyzed using the nucleotide sequence of the granulin gene, SlGV was most closely related to Trichoplusia ni granulovirus (TnGV) and Xestia c-nigrum granulovirus (XcGV) which belong to Type I granulovirus.
Keywordsbaculovirus Spodoptera litura granulovirus SlGV granulin
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- Federici, B.A. 1993. Viral pathobiology in relation to insect control, p. 21–101. In N.E. Beckage, S.N. Thompson, and B.A. Federici (eds.). Parasites and pathogens of Insects, Vol. 2, Pathogens. Academic Press, San Diego, USA.Google Scholar
- Federici, B.A. 1997. Baculoviral pathogenesis, p. 35–59. In L.K. Miller (ed.). The Baculoviruses. Plenum Press, New York, N.Y., USA.Google Scholar
- Huger, A. 1963. Granuloses of insects, p. 531–575. In E.A. Steinhaus (ed.). Insect Pathology: An Advanced Treatise. Vol. 1. America Press, New York, N.Y., USA.Google Scholar
- Martignoni, M.E. and P.J. Iwai. 1981. A catalogue of viral diseases of insects, mites and ticks, p. 897–911. In H.D. Burges (ed.). Microbial Control of Pests and Plant Diseases 1970–1980. Academic Press, London, UK.Google Scholar
- Murphy, F.A., C.M. Fauquet, D.H.L. Bishop, S.A. Ghabrial, A.W. Jarvis, G.P. Martinelli, M.A. Mayo, and M.D. Summers. 1995. Virus Taxonomy. Sixth report of the international committee on taxonomy of viruses. Arch. Virol. Suppl. 10, 85–86.Google Scholar
- O’Reilly, D.R., L.K. Miller, and V.A. Luckow. 1992. Baculovirus expression vectors: a laboratory manual. W. H. Freeman and Company, New York, N.Y., USA.Google Scholar
- Swfford, D.L. 2003. PAUP*. Phylogenetic Analysis Using Parsimony (*and Other Methods) Version 4. Sinauer Associates, Sunderland, Massachuertts, MA, USA (on disk).Google Scholar
- Theilmann, D.A., G.W. Blissard, B. Bonning, J.A. Jehle, D.R. O’Reilly, G.F. Rohrmann, S. Thiem, and J.M. Vlak. 2005. Baculovirudae, p. 177–185. In C.X. Fauquet, M.A. Mayo, J. Maniloff, U. Desselberger, and L.A. Ball (eds.). Virus Taxonomy-Classification and Nomenclature of Viruses. 8th Report of the International Committee on the Taxnomy of Viruses. Elsevier, Amsterdam, Netherland.Google Scholar
- Valk, J.M. and G.E. Smith. 1982. Orientation of the genome of Autographa californica nuclear polyhedrosis virus: a proposal. J. Virol. 41, 1118–1121.Google Scholar