Abstract
The expression of amoeba sams genes is switched from sams1 to sams2 when amoebae are infected with Legionella jeonii. To elucidate the mechanism for the inactivation of host sams1 gene by endosymbiotic bacteria, methylation states of the sams1 gene of D and xD amoebae was compared in this study. The sams1 gene of amoebae was methylated at an internal adenine residue of GATC site in symbiont-bearing xD amoebae but not in symbiont-free D amoebae, suggesting that the modification might have caused the inactivation of sams1 in xD amoebae. The sams1 gene of xD amoebae was inactivated at the transcriptional level. Analysis of DNA showed that adenine residues in L. jeonii sams were also methylated, implying that L. jeonii bacteria belong to a Dam methylase-positive strain. In addition, both SAM and Met appeared to act as negative regulators for the expression of sams1 whereas the expression of sams2 was not affected in amoebae.
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References
Bhagwat, A.S. and M. Lieb. 2002. Cooperation and competition in mismatch repair: very short-patch repair and methyl-directed mismatch repair in Escherichia coli. Mol. Microbiol. 44, 1421–1428.
Choi, E.Y. and K.W. Jeon. 1989. A spectrin-like protein present on membranes of Amoeba proteus as studied with monoclonal antibodies. Exp. Cell Res. 185, 154–165.
Choi, J.Y., T.W. Lee, K.W. Jeon, and T.I. Ahn. 1997. Evidence for symbiont-induced alteration of a host’s gene expression: Irreversible loss of SAM synthetase from Amoeba proteus. J. Euk. Microbiol. 44, 412–419.
Dehio, C. and J. Schell. 1994. Identification of plant genetic loci involved in a posttranscriptional mechanism for meiotically reversible transgene silencing. Proc. Natl. Acad. Sci. USA 91, 5538–5542.
Ghoshal, K., S. Majumder, Z. Li, X. Dong, and S.T. Jacob. 2000. Suppression of metallothionein gene expression in a rat hepatoma because of promoter-specific DNA methylation. J. Biol. Chem. 275, 539–547.
Goldstein, L. and C. Ko. 1976. A method for the mass culturing of large free-living amebas. Methods Cell Biol. 13, 239–246.
Hattman, S. 2005. DNA-[Adenine] methylation in lower eukaryotes. Biochem. (Moscow) 70, 550–558.
Heithoff, D.M., R.L. Sinsheimer, D.A. Low, and M.J. Mahan. 1999. An essential role for DNA adenine methylation in bacterial virulence. Science 284, 967–970.
Heusipp, G., S. Falker, and M.A. Schmidt. 2007. DNA adenine methylation and bacterial pathogenesis. Int. J. Med. Microbiol. 297, 1–7.
Ichida, H., T. Matsuyama, T. Abe, and T. Koba. 2007. DNA adenine methylation changes dramatically during establishment of symbiosis. FEBS J. 274, 951–962.
Jeon, K.W. 2004. Genetic and physiological interactions in the amoeba-bacteria symbiosis. J. Eukaryot. Microbiol. 51, 502–508.
Jeon, K.W. and T.I. Ahn. 1978. Temperature sensitivity: A cell character determined by obligate endosymbionts in Amoebas. Science 202, 635–637.
Jeon, K.W. and M.S. Jeon. 1975. Cytoplasmic filaments and cellular wound healing in Amoeba proteus. J. Cell Biol. 67, 243–249.
Jeon, T.J. and K.W. Jeon. 2003. Characterization of sams genes of Amoeba proteosamsus and the endosymbiotic X-bacteria. J. Eukaryot. Microbiol. 50, 61–69.
Jeon, T.J. and K.W. Jeon. 2004. Gene switching in Amoeba proteus caused by endosymbiotic bacteria. J. Cell Sci. 117, 535–543.
Jeon, K.W. and I.J. Lorch. 1967. Unusual intra-cellular bacterial infection in large, free-living amoebae. Exp. Cell. Res. 48, 236–240.
Lee, M.P. 2003. Genome-wide analysis of epigenetics in cancer. Ann. N Y Acad. Sci. 983, 101–109.
Lema, M.W. and A. Brown. 1996. Dam-like methylation in Legionellae. Curr. Microbiol. 32, 64–66.
Low, D.A., N.J. Weyand, and M.J. Mahan. 2001. Roles of DNA adenine methylation in regulating bacterial gene expression and virulence. Infect. Immun. 69, 7197–7204.
Mato, J.M., F.J. Corrales, and M.A. Avila. 2002. S-Adenosylmethionine: a control switch that regulates liver function. FASEB J. 16, 15–26.
Mato, J.M. and S.C. Lu. 2007. Role of S-Adenosylmethionine in liver health and injury. Hepatology 45, 1306–1312.
Moores, S.L., J.H. Sabry, and J.S. Spudich. 1996. Myosin dynamics in live Dictyostelium cells. PNAS 93, 443–446.
Oh, S.W. and K.W. Jeon. 1998. Characterization of myosin heavy chain and its gene in Amoeba proteus. J. Eukaryot. Microbiol. 45, 600–605.
Oshima, T., C. Wada, W. Kawagoe, T. Ara, M. Maeda, Y. Masuda, S. Hiraga, and H. Mori. 2002. Genome-wide analysis of deoxyadenosine methyltransferase-mediated control of gene expression in Escherichia coli. Mol. Microbiol. 45, 673–695.
Pak, J.W. and K.W. Jeon. 1996. The s29x gene of symbiotic bacteria in Amoeba proteus with a novel promoter. Gene 171, 89–93.
Pak, J.W. and K.W. Jeon. 1997. A symbiont-produced protein and bacterial symbiosis in Amoeba proteus. J. Eukaryot. Microbiol. 44, 614–619.
Park, M., S.T. Yun, M.S. Kim, J. Chun, and T.I. Ahn. 2004. Phylogenetic characterization of Legionella-like endosymbiotic X-bacteria in Amoeba proteus: a proposal for ‘Candidatus Legionella jeonii’ sp. nov. Environ. Microbiol. 6, 1252–1263.
Shapiro, D.J., P.A. Sharp, W. Wahli, and M.J. Keller. 1988. A high efficiency Hela cells nuclear transcription extract. DNA 7, 47–55.
Thomas, D. and Y. Surdin-Kerjan. 1997. Metabolism of sulfur amino acids in Saccharomyces cerevisiae. Microbiol. Mol. Biol. Rev. 61, 503–532.
Vanyushin, B.F. 2006. DNA methylation and epigenetics. Russ. J. Genet. 42, 985–997.
Wion, D. and J. Casadesus. 2006. N6-methyl-adenine: an epigenetic signal for DNA-protein interactions. Nat. Rev. Microbiol. 4, 183–192.
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Jeon, T.J. DNA adenine methylation of sams1 gene in symbiont-bearing Amoeba proteus . J Microbiol. 46, 564–570 (2008). https://doi.org/10.1007/s12275-008-0129-8
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DOI: https://doi.org/10.1007/s12275-008-0129-8