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The Journal of Microbiology

, Volume 46, Issue 2, pp 174–182 | Cite as

Bacterial communities in the initial stage of marine biofilm formation on artificial surfaces

  • Jin-Woo Lee
  • Ji-Hyun Nam
  • Yang-Hoon Kim
  • Kyu-Ho Lee
  • Dong-Hun LeeEmail author
Articles

Abstract

Succession of bacterial communities during the first 36 h of biofilm formation in coastal water was investigated at 3∼15 h intervals. Three kinds of surfaces (i.e., acryl, glass, and steel substratum) were submerged in situ at Sacheon harbor, Korea. Biofilms were harvested by scraping the surfaces, and the compositions of bacterial communities were analyzed by terminal restriction fragment length polymorphism (T-RFLP), and cloning and sequencing of 16S rRNA genes. While community structure based on T-RFLP analysis showed slight differences by substratum, dramatic changes were commonly observed for all substrata between 9 and 24 h. Identification of major populations by 16S rRNA gene sequences indicated that γ-Proteobacteria (Pseudomonas, Acinetobacter, Alteromonas, and uncultured γ-Proteobacteria) were predominant in the community during 0∼9 h, while the ratio of α-Proteobacteria (Loktanella, Methylobacterium, Pelagibacter, and uncultured α-Proteobacteria) increased 2.6∼4.8 folds during 24∼36 h of the biofilm formation, emerging as the most predominant group. Previously, α-Proteobacteria were recognized as the pioneering organisms in marine biofilm formation. However, results of this study, which revealed the bacterial succession with finer temporal resolution, indicated some species of γ-Proteobacteria were more important as the pioneering population. Measures to control pioneering activities of these species can be useful in prevention of marine biofilm formation.

Keywords

16S rRNA bacterial community marine biofilm succession T-RFLP 

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Copyright information

© The Microbiological Society of Korea and Springer-Verlag Berlin Heidelber GmbH 2008

Authors and Affiliations

  • Jin-Woo Lee
    • 1
  • Ji-Hyun Nam
    • 1
  • Yang-Hoon Kim
    • 1
  • Kyu-Ho Lee
    • 2
  • Dong-Hun Lee
    • 1
    Email author
  1. 1.Department of MicrobiologyChungbuk National UniversityChungbukRepublic of Korea
  2. 2.Department of Environmental ScienceHankuk University of Foreign StudiesKyunggi-DoRepublic of Korea

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