The Journal of Microbiology

, Volume 46, Issue 1, pp 40–44

Arthrobacter soli sp. nov., a novel bacterium isolated from wastewater reservoir sediment

  • Seong Woon Roh
  • Youlboong Sung
  • Young-Do Nam
  • Ho-Won Chang
  • Kyoung-Ho Kim
  • Jung-Hoon Yoon
  • Che Ok Jeon
  • Hee-Mock Oh
  • Jin-Woo Bae
Article

Abstract

A novel Gram-positive bacterium, designated SYB2T, was isolated from wastewater reservoir sediment, and a polyphasic taxonomic study was conducted based on its morphological, physiological, and biochemical features, as well as the analysis of its 16S rRNA gene sequence. During the phylogenetic analysis of the strain SYB2T, results of a 16S rRNA gene sequence analysis placed this bacterium in the genus Arthrobacter within the family Micrococcaceae. SYB2T and Arthrobacter protophormiae ATCC 19271T, the most closely related species, both exhibited a 16S rRNA gene sequence similarity of 98.99%. The genomic DNA G+C content of the novel strain was found to be 62.0 mol%. The predominant fatty acid composition was ante-iso-C15:0, anteiso-C17:0, iso-C16:0, and iso-C15:0. Analysis of 16S rRNA gene sequences and DNA-DNA related-ness, as well as physiological and biochemical tests, showed genotypic and phenotypic differences between strain SYB2T and other Arthrobacter species. The type strain of the novel species was identified as SYB2T (= KCTC 19291T= DSM 19449T).

Keywords

Arthrobacter soli sp. nov taxonomy wastewater reservoir sediment 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Buck, J.D. 1982. Nonstaining (KOH) method for determination of Gram reactions of marine bacteria. Appl. Environ. Microbiol. 44, 992–993.PubMedGoogle Scholar
  2. Chen, M., X. Xiao, P. Wang, X. Zeng, and F. Wang. 2005. Arthrobacter ardleyensis sp. nov., isolated from Antarctic lake sediment and deep-sea sediment. Arch. Microbiol. 183, 301–305.PubMedCrossRefGoogle Scholar
  3. Chun, J., J.H. Lee, Y. Jung, M. Kim, S. Kim, B.K. Kim, and Y.W. Lim. 2007. EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int. J. Syst. Evol. Microbiol. 57, 2259–2261.PubMedCrossRefGoogle Scholar
  4. Collins, M.D., L. Hoyles, G. Foster, E. Falsen, and N. Weiss 2002. Arthrobacter nasiphocae sp. nov., from the common seal (Phoca vitulina). Int. J. Syst. Evol. Microbiol. 52, 569–571.PubMedGoogle Scholar
  5. Conn, H.J. 1928. A type of bacteria abundant in productive soils, but apparently lacking in certain soils of low productivity. NY. Agric. Exp. Stn. Geneva Bull. 138, 3–26.Google Scholar
  6. Conn, J. and I. Dimmick. 1947. Soil bacteria similar in morphology to Mycobacterium and Corynebacterium. J. Bacteriol. 54, 291–303.PubMedGoogle Scholar
  7. Ezaki, T., H. Hashimoto, and E. Yabuuchi. 1989. Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int. J. Syst. Bacteriol. 39, 224–229.Google Scholar
  8. Felsenstein, J. 1985. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.CrossRefGoogle Scholar
  9. Funke, G., R.A. Hutson, K.A. Bernard, G.E. Pfyffer, G. Wauters, and M.D. Collins. 1996. Isolation of Arthrobacter spp. from clinical specimens and description of Arthrobacter cumminsii sp. nov. and Arthrobacter woluwensis sp. nov. J. Clin. Microbiol. 34, 2356–2363.PubMedGoogle Scholar
  10. Gupta, P., G.S. Reddy, D. Delille, and S. Shivaji. 2004. Arthrobacter gangotriensis sp. nov. and Arthrobacter kerguelensis sp. nov. from Antarctica. Int. J. Syst. Evol. Microbiol. 54, 2375–2378.PubMedCrossRefGoogle Scholar
  11. Heyrman, J., J. Verbeeren, P. Schumann, J. Swings, and P. De Vos. 2005. Six novel Arthrobacter species isolated from deteriorated mural paintings. Int. J. Syst. Evol. Microbiol. 55, 1457–1464.PubMedCrossRefGoogle Scholar
  12. Hou, X.G., Y. Kawamura, F. Sultana, S. Shu, K. Hirose, K. Goto, and T. Ezaki. 1998. Description of Arthrobacter creatinolyticus sp. nov., isolated from human urine. Int. J. Syst. Bacteriol. 48, 423–429.PubMedGoogle Scholar
  13. Huang, Y., N. Zhao, L. He, L. Wang, Z. Liu, M. You, and F. Guan. 2005. Arthrobacter scleromae sp. nov. isolated from human clinical specimens. J. Clin. Microbiol. 43, 1451–1455.PubMedCrossRefGoogle Scholar
  14. Irlinger, F., F. Bimet, J. Delettre, M. Lefevre, and P.A. Grimont. 2005. Arthrobacter bergerei sp. nov. and Arthrobacter arilaitensis sp. nov., novel coryneform species isolated from the surfaces of cheeses. Int. J. Syst. Evol. Microbiol. 55, 457–462.PubMedCrossRefGoogle Scholar
  15. Kimura, M. 1980. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J. Mol. Evol. 16, 111–120.PubMedCrossRefGoogle Scholar
  16. Kotouckova, L., P. Schumann, E. Durnova, C. Sproer, I. Sedlacek, J. Neca, Z. Zdrahal, and M. Nemec. 2004. Arthrobacter nitroguajacolicus sp. nov., a novel 4-nitroguaiacol-degrading actinobacterium. Int. J. Syst. Evol. Microbiol. 54, 773–777.PubMedCrossRefGoogle Scholar
  17. Kumar, S., K. Tamura, and M. Nei. 2004. MEGA3: Integrated software for Molecular Evolutionary Genetics Analysis and sequence alignment. Brief Bioinform. 5, 150–163.PubMedCrossRefGoogle Scholar
  18. Lane, D.J. 1991. 16S/23S rRNA sequencing. In Nucleic acid techniques in bacterial systematics. John Wiley and Sons, New York, NY, USA.Google Scholar
  19. Lee, J.S., K.C. Lee, Y.R. Pyun, and K.S. Bae. 2003. Arthrobacter koreensis sp. nov., a novel alkalitolerant bacterium from soil. Int. J. Syst. Evol. Microbiol. 53, 1277–1280.PubMedCrossRefGoogle Scholar
  20. Li, Y., Y. Kawamura, N. Fujiwara, T. Naka, H. Liu, X. Huang, K. Kobayashi, and T. Ezaki. 2004. Rothia aeria sp. nov., Rhodococcus baikonurensis sp. nov. and Arthrobacter russicus sp. nov., isolated from air in the Russian space laboratory Mir. Int. J. Syst. Evol. Microbiol. 54, 827–835.PubMedCrossRefGoogle Scholar
  21. Margesin, R., P. Schumann, C. Sproer, and A.M. Gounot. 2004. Arthrobacter psychrophenolicus sp. nov., isolated from an alpine ice cave. Int. J. Syst. Evol. Microbiol. 54, 2067–2072.PubMedCrossRefGoogle Scholar
  22. Mesbah, M. and W.B. Whitman. 1989. Measurement of deoxyguanosine/thymidine ratios in complex mixtures by high-performance liquid chromatography for determination of the mole percentage guanine+cytosine of DNA. J. Chromatogr. 479, 297–306.PubMedCrossRefGoogle Scholar
  23. Osorio, C.R., J.L. Barja, R.A. Hutson, and M.D. Collins. 1999. Arthrobacter rhombi sp. nov., isolated from Greenland halibut (Reinhardtius hippoglossoides). Int. J. Syst. Bacteriol. 49, 1217–1220.PubMedGoogle Scholar
  24. Reddy, G.S., R.K. Aggarwal, G.I. Matsumoto, and S. Shivaji. 2000. Arthrobacter flavus sp. nov., a psychrophilic bacterium isolated from a pond in McMurdo Dry Valley, Antarctica. Int. J. Syst. Evol. Microbiol. 50, 1553–1561.PubMedGoogle Scholar
  25. Reddy, G.S., J.S. Prakash, G.I. Matsumoto, E. Stackebrandt, and S. Shivaji. 2002. Arthrobacter roseus sp. nov., a psychrophilic bacterium isolated from an antarctic cyanobacterial mat sample. Int. J. Syst. Evol. Microbiol. 52, 1017–1021.PubMedCrossRefGoogle Scholar
  26. Saitou, N. and M. Nei. 1987. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 4, 406–425.PubMedGoogle Scholar
  27. Sasser, M. 1990. Identification of bacteria by gas chromatography of cellular fatty acids. MIDI Technical Note No.101. Newark, DE: MIDI Inc., USA.Google Scholar
  28. Stackebrandt, E., V. Fowler, F. Fiedler, and H. Seiler. 1983. Taxonomic studies on Arthrobacter nicotianae and related Taxa: Description of Arthrobacter uratoxydans sp. nov. and Arthrobacter sulfurous sp. nov. and reclassification of Brevibacterium protophormiae as Arthrobacter protophormiae comb nov. Syst. Appl. Microbiol. 4, 470–486.Google Scholar
  29. Storms, V., L.A. Devriese, R. Coopman, P. Schumann, F. Vyncke, and M. Gillis. 2003. Arthrobacter gandavensis sp. nov., for strains of veterinary origin. Int. J. Syst. Evol. Microbiol. 53, 1881–1884.PubMedCrossRefGoogle Scholar
  30. Thompson, J.D., T.J. Gibson, F. Plewniak, F. Jeanmougin, and D.G. Higgins. 1997. The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res. 25, 4876–4882.PubMedCrossRefGoogle Scholar
  31. Tvrzova, L., P. Schumann, C. Sproer, I. Sedlacek, S. Verbarg, R.M. Kroppenstedt, and Z. Pacova. 2005. Polyphasic taxonomic study of strain CCM 2783 resulting in the description of Arthrobacter stackebrandtii sp. nov. Int. J. Syst. Evol. Microbiol. 55, 805–808.PubMedCrossRefGoogle Scholar
  32. Wauters, G., J. Charlier, M. Janssens, and M. Delmee. 2000. Identification of Arthrobacter oxydans, Arthrobacter luteolus sp. nov., and Arthrobacter albus sp. nov., isolated from human clinical specimens. J. Clin. Microbiol. 38, 2412–2415.PubMedGoogle Scholar
  33. Wayne, L.G., D.J. Brenner, and R.R. Colwell. 1987. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int. J. Syst. Bacteriol. 37, 463–464.CrossRefGoogle Scholar

Copyright information

© The Microbiological Society of Korea 2008

Authors and Affiliations

  • Seong Woon Roh
    • 1
    • 2
  • Youlboong Sung
    • 3
  • Young-Do Nam
    • 1
    • 2
  • Ho-Won Chang
    • 2
  • Kyoung-Ho Kim
    • 2
  • Jung-Hoon Yoon
    • 2
  • Che Ok Jeon
    • 4
  • Hee-Mock Oh
    • 2
  • Jin-Woo Bae
    • 1
    • 2
    • 4
  1. 1.Korea University of Science and TechnologyDaejeonRepublic of Korea
  2. 2.Biological Resource CenterKRIBBDaejeonRepublic of Korea
  3. 3.Environmental Research DepartmentResearch Institute of Industrial Science and TechnologyGwangyangRepublic of Korea
  4. 4.Environmental Biotechnology National Core Research CenterGyeongsang National UniversityJinjuRepublic of Korea

Personalised recommendations