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The Journal of Microbiology

, Volume 46, Issue 4, pp 429–435 | Cite as

Promoter analysis of Bombyx mori nucleopolyhedrovirus Ubiquitin gene

  • Xu’ai Lin
  • Yin Chen
  • Yongzhu Yi
  • Jie Yan
  • Zhifang Zhang
Articles

Abstract

The aim of this study was to analyze the characteristics of Bombyx mori nucleopolyhedrovirus (BmNPV) ubiquitin gene promoter and the effects of conserved motifs, such as TAAG, TATA, and CAAT, along with baculovirus enhancer homologous region 3 (hr3), on promoter activity. Ubiquitin gene of BmNPV was expressed during the late phase of virus infection. In the presence of viral factors, significant reduction of promoter activity was observed by deletion of −382 to −124 bp upstream of ATG. The fragment between −187 and −383 bp upstream of ATG, including distal TAAG, CAAT motif, and TATA box, could also drive expression of the reporter gene. The mutation of cis-elements TATA boxes and TAAG motifs significantly decreased the promoter’s activity, while CAAT mutations enhanced promoter activity by 2-or 3-fold, as compared with the native promoter. In the presence of BmNPV, hr3, both located downstream of the reporter gene of the same vector, and separate vector, could significantly enhance transcription activity of ubiquitin promoter as compared to the control. We concluded that BmNPV ubiquitin gene might be regulated by dual sets of promoter elements, where TAAG and TATA box may positively regulate the expression of ubiquitin, while CAAT motif functions as a negative regulator. Viral factor(s) play an important role in the co-activation of hr3 and promoter.

Keywords

baculovirus ubiquitin promoter transient expression mutation enhancer hr3 

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Copyright information

© The Microbiological Society of Korea and Springer-Verlag Berlin Heidelber GmbH 2008

Authors and Affiliations

  • Xu’ai Lin
    • 1
    • 2
  • Yin Chen
    • 2
    • 3
  • Yongzhu Yi
    • 2
  • Jie Yan
    • 1
  • Zhifang Zhang
    • 2
  1. 1.Department of Medical Microbiology and Parasitology, School of MedicineZhejiang UniversityHangzhouP. R. China
  2. 2.Biotechnology Research Institute, National Key Facility for Crop Gene Resources and Genetic ImprovementChinese Academy of Agricultural SciencesBeijingP. R. China
  3. 3.Zhejiang Provincial Center for Disease Control and PreventionHangzhouP. R. China

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