Carbon nanostructure morphology templates nanocomposites for phosphoproteomics


Protein and peptide phosphorylation regulate numerous pathological processes, however, their characterization is challenging due to their low abundance and transient nature. Therefore, nanomaterials are being developed to address this demanding task. In particular, carbon nanostructures are attracting interest as scaffolds for functional nanocomposites, yet only isolated studies exist on the topic, and little is known on the effect of nanocarbon morphology on templating nanocomposites. In this work, we compared oxidized carbon nanotubes, graphene oxide, oxidized carbon nanohorns and oxidized graphitized carbon black, as scaffolds for magnetized nanocomposites. The nanomaterials were extensively characterized with experimental and in silico techniques. Next, they were applied to phosphopeptide enrichment from cancer cell lysates for NanoHPLC-MS/MS, with selectivity as high as nearly 90% and several-thousand identification hits. Overall, new insights emerged for the understanding and the design of nanocomposites for phosphoproteomics.

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Change history

  • 14 January 2020

    The fourth author’s name was unfortunately misspelled on the first page and the first page of the ESM.

    Instead of Susy Piovesana<Superscript>1</Superscript>, Daniel Iglesias<Superscript>2</Superscript>, Manuel Melle-Franco<Superscript>3</Superscript>, Slavo Kralj<Superscript>4</Superscript>, Chiara Cavaliere<Superscript>1</Superscript>, Michele Melchionna<Superscript>2</Superscript>, Aldo Laganà<Superscript>1,5</Superscript>, Anna L. Capriotti<Superscript>1</Superscript> (✉), and Silvia Marchesan<Superscript>2</Superscript> (✉)

    It should read Susy Piovesana<Superscript>1</Superscript>, Daniel Iglesias<Superscript>2</Superscript>, Manuel Melle-Franco<Superscript>3</Superscript>, Slavko Kralj<Superscript>4</Superscript>, Chiara Cavaliere<Superscript>1</Superscript>, Michele Melchionna<Superscript>2</Superscript>, Aldo Laganà<Superscript>1,5</Superscript>, Anna L. Capriotti<Superscript>1</Superscript> (✉), and Silvia Marchesan<Superscript>2</Superscript> (✉)


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This work was supported by PRIN 2015, project number 2015TWP83Z. The authors would like to acknowledge networking support by the COST Action MultiComp CA15107, as well as financial support from the Slovenian Research Agency (ARRS) for research core funding No. (P2-0089) and projects No. (J1-7302 and J3-7494).

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Correspondence to Anna L. Capriotti or Silvia Marchesan.

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Piovesana, S., Iglesias, D., Melle-Franco, M. et al. Carbon nanostructure morphology templates nanocomposites for phosphoproteomics. Nano Res. (2020) doi:10.1007/s12274-020-2620-4

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  • nanocomposites
  • carbon nanotubes
  • graphene oxide
  • carbon nanohorns
  • proteomics
  • magnetic