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Efficient renal clearance of DNA tetrahedron nanoparticles enables quantitative evaluation of kidney function

  • Dawei Jiang
  • Hyung-Jun Im
  • Madeline E. Boleyn
  • Christopher G. England
  • Dalong Ni
  • Lei Kang
  • Jonathan W. Engle
  • Peng HuangEmail author
  • Xiaoli LanEmail author
  • Weibo CaiEmail author
Research Article
  • 25 Downloads

Abstract

DNA tetrahedron nanostructure (DTN) is one of the simplest DNA nanostructures and has been successfully applied for biosensing, imaging, and treatment of cancer. To facilitate its biomedical applications and potential clinical translation, fundamental understanding of DTN’s transportation among major organs in living organisms becomes increasingly important. Here, we describe the efficient renal clearance of DTN in healthy mice by using positron emission tomography (PET) imaging. The kidney elimination of DTN was later applied for renal function evaluation in murine models of unilateral ureteral obstruction (UUO). We further established a mathematical program of DTN to validate its changes of transportation pattern in healthy and UUO mice. We believe the establishment of pharmacokinetic profiles and mathematical model of DTN may provide insight for future optimization of DNA nanostructures for biomedical applications.

Keywords

positron emission tomography (PET) imaging DNA nanotechnology DNA tetrahedron nanoparticle renal clearance kidney dysfunction 

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Notes

Acknowledgements

This work was supported by the University of Wisconsin-Madison, the National Institutes of Health (NIBIB/NCI P30CA014520, T32CA009206), the American Cancer Society (125246-RSG-13-099-01-CCE), the National Natural Science Foundation of China (Nos. 51573096, 51703132, 31771036, and 81630049) and the Basic Research Program of Shenzhen (Nos. JCYJ20170412111100742 and JCYJ20160422091238319), the Guangdong Province Natural Science Foundation of Major Basic Research and Cultivation Project (No. 2018B030308003), and Fok Ying-Tong Education Foundation for Young Teachers in the Higher Education Institutions of China (No. 161032).

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Efficient renal clearance of DNA tetrahedron nanoparticles enables quantitative evaluation of kidney function
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Copyright information

© Tsinghua University Press and Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Dawei Jiang
    • 1
    • 2
  • Hyung-Jun Im
    • 1
    • 3
  • Madeline E. Boleyn
    • 1
    • 4
  • Christopher G. England
    • 1
  • Dalong Ni
    • 1
  • Lei Kang
    • 1
    • 5
  • Jonathan W. Engle
    • 1
  • Peng Huang
    • 2
    Email author
  • Xiaoli Lan
    • 6
    Email author
  • Weibo Cai
    • 1
    Email author
  1. 1.Departments of Radiology and Medical PhysicsUniversity of Wisconsin-MadisonMadisonUSA
  2. 2.Guangdong Key Laboratory for Biomedical Measurements and Ultrasound Imaging, Carson International Cancer Center, Laboratory of Evolutionary Theranostics, School of Biomedical Engineering, Health Science CenterShenzhen UniversityShenzhenChina
  3. 3.Graduate School of Convergence Science and TechnologySeoul National UniversitySeoulRepublic of Korea
  4. 4.Departments of Mathematics, and BiologyUniversity of Wisconsin-MadisonWisconsinUSA
  5. 5.Department of Nuclear MedicinePeking University First HospitalBeijingChina
  6. 6.Department of Nuclear Medicine, Union Hospital, Tongji Medical College, Huazhong University of Science and TechnologyHubei Key Laboratory of Molecular ImagingWuhanChina

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