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Nano Research

, Volume 11, Issue 8, pp 4005–4016 | Cite as

Surface-enhanced Raman scattering nanosensors for in vivo detection of nucleic acid targets in a large animal model

  • Hsin-Neng Wang
  • Janna K. Register
  • Andrew M. Fales
  • Naveen Gandra
  • Eugenia H. Cho
  • Alina Boico
  • Gregory M. Palmer
  • Bruce Klitzman
  • Tuan Vo-Dinh
Research Article

Abstract

Although nanotechnology has led to important advances in in vitro diagnostics, the development of nanosensors for in vivo detection remains very challenging. Here, we demonstrated the proof-of-principle of in vivo detection of nucleic acid targets using a promising type of surface-enhanced Raman scattering (SERS) nanosensor implanted in the skin of a large animal model (pig). The in vivo nanosensor used in this study involves the “inverse molecular sentinel” detection scheme using plasmonics-active nanostars, which have tunable absorption bands in the near infrared region of the “tissue optical window”, rendering them efficient as an optical sensing platform for in vivo optical detection. Ex vivo measurements were also performed using human skin grafts to demonstrate the detection of SERS nanosensors through tissue. In this study, a new core–shell nanorattle probe with Raman reporters trapped between the core and shell was utilized as an internal standard system for self-calibration. These results illustrate the usefulness and translational potential of the SERS nanosensor for in vivo biosensing.

Keywords

nanosensor nanoprobes plasmonics nanostar surface-enhanced Raman scattering (SERS) in vivo sensing 

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Notes

Acknowledgements

This work was sponsored by the Defense Advanced Research Projects Agency (No. HR0011-13-2-0003). The content of the information does not necessarily reflect the position or the policy of the Government, and no official endorsement should be inferred.

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Copyright information

© Tsinghua University Press and Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Hsin-Neng Wang
    • 1
    • 2
  • Janna K. Register
    • 1
    • 2
  • Andrew M. Fales
    • 1
    • 2
  • Naveen Gandra
    • 1
    • 2
  • Eugenia H. Cho
    • 3
  • Alina Boico
    • 3
  • Gregory M. Palmer
    • 3
  • Bruce Klitzman
    • 3
  • Tuan Vo-Dinh
    • 1
    • 2
    • 4
  1. 1.Departments of Biomedical EngineeringDuke UniversityDurhamUSA
  2. 2.Fitzpatrick Institute for PhotonicsDuke UniversityDurhamUSA
  3. 3.Medical CenterDuke UniversityDurhamUSA
  4. 4.Departments of ChemistryDuke UniversityDurhamUSA

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