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Nano Research

, Volume 5, Issue 7, pp 450–459 | Cite as

Easy access to selective binding and recyclable separation of histidine-tagged proteins using Ni2+-decorated superparamagnetic nanoparticles

  • Zhen Liu
  • Meng Li
  • Zhenhua Li
  • Fang Pu
  • Jinsong RenEmail author
  • Xiaogang Qu
Research Article

Abstract

The development of simple techniques for the separation and purification of recombinant proteins plays an important role in many of the advancements made in biotechnology and nanotechnology. Herein, we report an easy method for the efficient purification of polyhistidine affinity-tagged (His-tagged) proteins by using Ni2+-decorated superparamagnetic particles. Monodisperse Ni0.3Fe0.7Fe2O4 nanoparticles were prepared via a facile and economical one-pot hydrothermal process. Owing to the characteristic molecular recognition ability between nickel(II) ions and the polyhistidine affinity tag, the nanoparticles could be successfully employed to selectively bind and separate His-tagged cyan fluorescent protein (CFP) from an E. coli cell lysate in a recyclable process. Moreover, by changing the divalent metal precursors, various other metal-decorated magnetic nanoparticles can be obtained. This approach offers the possibility of constructing metal-decorated nanoparticles through a simple method and will be highly beneficial in further applications of nanoparticle-based technologies.

Keywords

Magnetic nanoparticles green synthesis protein purification recyclable process biotechnology 

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Copyright information

© Tsinghua University Press and Springer-Verlag Berlin Heidelberg 2012

Authors and Affiliations

  • Zhen Liu
    • 1
    • 2
  • Meng Li
    • 1
    • 2
  • Zhenhua Li
    • 1
    • 2
  • Fang Pu
    • 1
  • Jinsong Ren
    • 1
    Email author
  • Xiaogang Qu
    • 1
  1. 1.State Key Laboratory of Rare Earth Resources Utilization and Laboratory of Chemical Biology, Changchun Institute of Applied ChemistryChinese Academy of SciencesChangchunChina
  2. 2.Graduate School of the Chinese Academy of SciencesBeijingChina

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