Archives of Pharmacal Research

, Volume 42, Issue 8, pp 722–731 | Cite as

Isoorientin improves scopolamine-induced cognitive impairments by restoring the cholinergic system, antioxidant defense, and p-CREB/BDNF signaling in the hippocampus and frontal cortex

  • Yong-Hyun Ko
  • Seung-Hwan Kwon
  • Seok-Yong Lee
  • Choon-Gon JangEmail author
Research Article


Isoorientin (ISO) is considered one of the most important flavonoids with various pharmacological effects such as antioxidant, anti-inflammatory, and anti-cancer activities. Despite these beneficial activities, the effects of ISO on learning and memory have not been investigated so far. The current study evaluated the memory-enhancing effects of ISO in a scopolamine-treated mouse model by using the Y-maze and passive avoidance tests. The results showed that ISO (5 and 10 mg/kg, p.o.) treatment significantly improved the cognitive impairments caused by scopolamine. Additionally, ISO significantly decreased scopolamine-induced acetylcholinesterase and thiobarbituric acid reactive substance activities in both the hippocampus and frontal cortex of mice. In addition, ISO significantly increased the levels of total superoxide dismutase induced by scopolamine in the hippocampus and frontal cortex. Moreover, Western blot results indicated that ISO reversed the decreases in expression of phosphorylated cAMP response element binding (CREB) and brain-derived neurotrophic factor (BDNF) in the hippocampus and frontal cortex of scopolamine-treated mice. Thus, our results provide initial evidence that ISO ameliorates scopolamine-induced memory and cognitive impairments partly by restoring the cholinergic system, antioxidant defense, and p-CREB/BDNF signaling pathway, thereby exhibiting memory-enhancing activities.


Isoorientin Cognitive function Cholinergic system Brain-derived neurotrophic factor cAMP response element binding Alzheimer’s disease 



This research was supported by Grants (NRF-2017R1A2B2002428) from the Basic Science Research Program through the National Research Foundation of Korea (NRF).

Compliance with ethical standards

Conflict of interest

The authors state that they have no conflicts of interest.


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Copyright information

© The Pharmaceutical Society of Korea 2019

Authors and Affiliations

  • Yong-Hyun Ko
    • 1
  • Seung-Hwan Kwon
    • 1
  • Seok-Yong Lee
    • 1
  • Choon-Gon Jang
    • 1
    Email author
  1. 1.Department of Pharmacology, School of PharmacySungkyunkwan UniversitySuwonRepublic of Korea

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