Archives of Pharmacal Research

, Volume 42, Issue 6, pp 455–465 | Cite as

Various approaches for measurement of synaptic vesicle endocytosis at the central nerve terminal

  • Yeonsun Jin
  • Kyoung Hee Seo
  • Hyun Myung Ko
  • Tae Woo Jung
  • Yoon Hee Chung
  • Jong Hyuk Lee
  • Hyun Ho Park
  • Hyoung-Chun Kim
  • Ji Hoon Jeong
  • Sung Hoon LeeEmail author


At the presynaptic terminal, neurotransmitters are stored in synaptic vesicles (SVs), which are released and recycled via exo- and endocytosis. SV endocytosis is crucial for sustaining synaptic transmission by maintaining the SV pool. Many studies have shown that presynaptic dysfunction, particularly impairment of SV endocytosis, is related to neurological disorders. Notably, the presynaptic terminal is considered to be a sensitive structure because certain presynaptic dysfunctions, manifested as impaired SV endocytosis or ultrastructural changes in the presynaptic terminal, can be observed before there is a biochemical or pathological evidence of a neurological disorder. Therefore, monitoring and assessing the presynaptic function by SV endocytosis facilitates the development of early markers for neurological disorders. In this study, we reviewed the current methods for assessing and visualizing SV endocytosis at the central nerve terminal.


Presynaptic terminal Synaptic vesicle Endocytosis Method Central nerve terminal 



This research was supported by the National Research Foundation of Korea (NRF), Grant funded by the Korea Government (MEST) (No. 2017R1D1A1B03031920), and the Chung-Ang University Graduate Research Scholarship in 2018.

Compliance with ethical standards

Conflicts of interest

The authors declare no conflict of interest.


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Copyright information

© The Pharmaceutical Society of Korea 2019

Authors and Affiliations

  • Yeonsun Jin
    • 1
  • Kyoung Hee Seo
    • 1
  • Hyun Myung Ko
    • 2
  • Tae Woo Jung
    • 3
  • Yoon Hee Chung
    • 4
  • Jong Hyuk Lee
    • 5
  • Hyun Ho Park
    • 1
  • Hyoung-Chun Kim
    • 6
  • Ji Hoon Jeong
    • 7
  • Sung Hoon Lee
    • 1
    Email author
  1. 1.College of PharmacyChung-Ang UniversitySeoulRepublic of Korea
  2. 2.Department of Life Science, College of Science and TechnologyWoosuk UniversityJincheonRepublic of Korea
  3. 3.Research Administration TeamSeoul National University Bundang HospitalSeongnamKorea
  4. 4.Department of Anatomy, College of MedicineChung-Ang UniversitySeoulRepublic of Korea
  5. 5.Department of Pharmaceutical Engineering, College of Life and Health ScienceHoseo UniversityAsanRepublic of Korea
  6. 6.Neuropsychopharmacology and Toxicology Program, College of PharmacyKangwon National UniversityChunchonRepublic of Korea
  7. 7.Department of Pharmacology, College of MedicineChung-Ang UniversitySeoulRepublic of Korea

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