Archives of Pharmacal Research

, Volume 40, Issue 9, pp 1087–1093 | Cite as

Effect of processing method on platycodin D content in Platycodon grandiflorum roots

  • Jaeyoung Kwon
  • Hyaemin Lee
  • Nahyun Kim
  • Je-Hyun Lee
  • Mi Hee Woo
  • Jinwoong Kim
  • Yeong Shik Kim
  • Dongho LeeEmail author
Research Article


Platycodon grandiflorum root is a traditional medicine and food material rich in triterpenoid saponins. Its major constituent, platycodin D (PD), is known to have various pharmacological properties, but processing methods may influence the PD content. In this study, a fully validated HPLC–ELSD method was developed for the quantification of PD in various states of 73 P. grandiflorum root samples from East Asia, and it exhibited a marked variation of the content. Furthermore, the effects of processing procedures such as peeling and drying temperature on the PD content were investigated using UPLC–ELSD analysis, and as a result, a significant influence of processing methods such as peeling and heating of samples on the content was confirmed. Specifically, unpeeled samples that were dried at 40 °C showed the greatest PD content. The obtained results could facilitate the reliable standardization of P. grandiflorum for precise authentication and efficacious applications.


Platycodon grandiflorum Platycodi Radix Platycodin D HPLC–ELSD Processing method 



This work was supported by a grant from the National Research Foundation of Korea (NRF-2015R1D1A1A01060321).

Compliance with ethical standards

Conflict of interest

All authors have no conflict of interest to declare.

Supplementary material

12272_2017_946_MOESM1_ESM.docx (100 kb)
Supplementary material 1 (DOCX 100 kb)


  1. Ahn KS, Noh EJ, Zhao HL, Jung SH, Kang SS, Kim YS (2005) Inhibition of inducible nitric oxide synthase and cyclooxygenase II by Platycodon grandiflorum saponins via suppression of nuclear factor-nB activation in RAW 264.7 cells. Life Sci 76:2315–2328CrossRefPubMedGoogle Scholar
  2. Chen T, Gao J, Xiang P, Chen Y, Ji J, Xie P, Wu H, Xiao W, Wei Y, Wang S (2015) Protective effect of platycodin D on liver injury in alloxan-induced diabetic mice via regulation of Treg/Th17 balance. Int Immunopharmacol 26:338–348CrossRefPubMedGoogle Scholar
  3. Cordell GA, Colvard MD (2012) Natural products and traditional medicine: turning on a paradigm. J Nat Prod 75:514–525CrossRefPubMedGoogle Scholar
  4. FDA (2013) Guidance for industry: bioanalytical method validation. Accessed 11 Aug 2016
  5. Han LK, Zheng YN, Xu BJ, Okuda H, Kimura Y (2002) Saponins from Platycodi Radix ameliorate high fat diet-induced obesity in mice. J Nutr 132:2241–2245PubMedGoogle Scholar
  6. Higashi A, Arimoto K, Iwata Y, Ujita K, Kawai T, Kobayashi Y, Sakurai K, Shimada Y, Takagi A, Taniyama T, Nakajima KI, Hisata Y, Hosoda K, Yamamoto K, Yamamoto Y, Noguchi M (1997) Studies on the effect of preparation methods of Platycodon root on its quality. Nat Med 51:56–62Google Scholar
  7. ICH (2005) ICH harmonised tripartite guideline: validation of analytical procedures. Accessed Nov 2005
  8. Jiang Y, David B, Tu P, Barbin Y (2010) Recent analytical approaches in quality control of traditional Chinese medicines—a review. Anal Chim Acta 657:9–18CrossRefPubMedGoogle Scholar
  9. Kim N, Ryu SM, Lee D, Lee JW, Seo EK, Lee JH, Lee D (2014) A metabolomic approach to determine the geographical origins of Anemarrhena asphodeloides by using UPLC–QTOF MS. J Pharm Biomed Anal 92:47–52CrossRefPubMedGoogle Scholar
  10. Kwon J, Kim N, Lee D, Han AR, Lee JW, Seo EK, Lee JH, Lee D (2014) Metabolomics approach for the discrimination of raw and steamed Gastrodia elata using liquid chromatography quadrupole time-of-flight mass spectrometry. J Pharm Biomed Anal 94:132–138CrossRefPubMedGoogle Scholar
  11. Lee B, Jeon S, Lee S, Chun H, Cho Y (2014) Effect of drying methods on the saponin and mineral contents of Platycodon grandiflorum Radix. Korean J Food Sci Technol 46:636–640CrossRefGoogle Scholar
  12. Luan X, Gao YG, Guan YY, Xu JR, Lu Q, Zhao M, Liu YR, Liu HJ, Fang C, Chen HZ (2014) Platycodin D inhibits tumor growth by antiangiogenic activity via blocking VEGFR2-mediated signaling pathway. Toxicol Appl Pharmacol 281:118–124CrossRefPubMedGoogle Scholar
  13. Wu J, Yang G, Zhu W, Wen W, Zhang F, Yuan J, An L (2012) Anti-atherosclerotic activity of platycodin D derived from roots of Platycodon grandiflorum in human endothelial cells. Biol Pharm Bull 35:1216–1221CrossRefPubMedGoogle Scholar
  14. Yoo DS, Choi YH, Cha MR, Lee BH, Kim SJ, Yon GH, Hong KS, Jang YS, Lee HS, Kim YS, Ryu SY, Kang JS (2011) HPLC-ELSD analysis of 18 platycosides from balloon flower roots (Platycodi Radix) sourced from various regions in Korea and geographical clustering of the cultivation areas. Food Chem 129:645–651CrossRefGoogle Scholar
  15. Zhang T, Yang S, Du J, Jinfu Y, Shumin W (2015) Platycodin D attenuates airway inflammation in a mouse model of allergic asthma by regulation NF-κB pathway. Inflammation 38:1221–1228CrossRefPubMedGoogle Scholar

Copyright information

© The Pharmaceutical Society of Korea 2017

Authors and Affiliations

  • Jaeyoung Kwon
    • 1
  • Hyaemin Lee
    • 2
  • Nahyun Kim
    • 3
  • Je-Hyun Lee
    • 4
  • Mi Hee Woo
    • 5
  • Jinwoong Kim
    • 6
  • Yeong Shik Kim
    • 7
  • Dongho Lee
    • 2
    Email author
  1. 1.Natural Constituents Research CenterKorea Institute of Science and Technology (KIST) Gangneung InstituteGangneungKorea
  2. 2.Department of Biosystems and Biotechnology, College of Life Sciences and BiotechnologyKorea UniversitySeoulKorea
  3. 3.Forest Medicinal Resources Research CenterNational Institute of Forest ScienceYeongjuKorea
  4. 4.College of Oriental MedicineDongguk UniversityGyeongjuKorea
  5. 5.College of PharmacyCatholic University of DaeguGyeongsanKorea
  6. 6.College of Pharmacy and Research Institute of Pharmaceutical SciencesSeoul National UniversitySeoulKorea
  7. 7.Natural Products Research Institute, College of PharmacySeoul National UniversitySeoulKorea

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