Effect of processing method on platycodin D content in Platycodon grandiflorum roots
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Platycodon grandiflorum root is a traditional medicine and food material rich in triterpenoid saponins. Its major constituent, platycodin D (PD), is known to have various pharmacological properties, but processing methods may influence the PD content. In this study, a fully validated HPLC–ELSD method was developed for the quantification of PD in various states of 73 P. grandiflorum root samples from East Asia, and it exhibited a marked variation of the content. Furthermore, the effects of processing procedures such as peeling and drying temperature on the PD content were investigated using UPLC–ELSD analysis, and as a result, a significant influence of processing methods such as peeling and heating of samples on the content was confirmed. Specifically, unpeeled samples that were dried at 40 °C showed the greatest PD content. The obtained results could facilitate the reliable standardization of P. grandiflorum for precise authentication and efficacious applications.
KeywordsPlatycodon grandiflorum Platycodi Radix Platycodin D HPLC–ELSD Processing method
This work was supported by a grant from the National Research Foundation of Korea (NRF-2015R1D1A1A01060321).
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Conflict of interest
All authors have no conflict of interest to declare.
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