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Archives of Pharmacal Research

, Volume 40, Issue 9, pp 1087–1093 | Cite as

Effect of processing method on platycodin D content in Platycodon grandiflorum roots

  • Jaeyoung Kwon
  • Hyaemin Lee
  • Nahyun Kim
  • Je-Hyun Lee
  • Mi Hee Woo
  • Jinwoong Kim
  • Yeong Shik Kim
  • Dongho LeeEmail author
Research Article

Abstract

Platycodon grandiflorum root is a traditional medicine and food material rich in triterpenoid saponins. Its major constituent, platycodin D (PD), is known to have various pharmacological properties, but processing methods may influence the PD content. In this study, a fully validated HPLC–ELSD method was developed for the quantification of PD in various states of 73 P. grandiflorum root samples from East Asia, and it exhibited a marked variation of the content. Furthermore, the effects of processing procedures such as peeling and drying temperature on the PD content were investigated using UPLC–ELSD analysis, and as a result, a significant influence of processing methods such as peeling and heating of samples on the content was confirmed. Specifically, unpeeled samples that were dried at 40 °C showed the greatest PD content. The obtained results could facilitate the reliable standardization of P. grandiflorum for precise authentication and efficacious applications.

Keywords

Platycodon grandiflorum Platycodi Radix Platycodin D HPLC–ELSD Processing method 

Notes

Acknowledgements

This work was supported by a grant from the National Research Foundation of Korea (NRF-2015R1D1A1A01060321).

Compliance with ethical standards

Conflict of interest

All authors have no conflict of interest to declare.

Supplementary material

12272_2017_946_MOESM1_ESM.docx (100 kb)
Supplementary material 1 (DOCX 100 kb)

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Copyright information

© The Pharmaceutical Society of Korea 2017

Authors and Affiliations

  • Jaeyoung Kwon
    • 1
  • Hyaemin Lee
    • 2
  • Nahyun Kim
    • 3
  • Je-Hyun Lee
    • 4
  • Mi Hee Woo
    • 5
  • Jinwoong Kim
    • 6
  • Yeong Shik Kim
    • 7
  • Dongho Lee
    • 2
    Email author
  1. 1.Natural Constituents Research CenterKorea Institute of Science and Technology (KIST) Gangneung InstituteGangneungKorea
  2. 2.Department of Biosystems and Biotechnology, College of Life Sciences and BiotechnologyKorea UniversitySeoulKorea
  3. 3.Forest Medicinal Resources Research CenterNational Institute of Forest ScienceYeongjuKorea
  4. 4.College of Oriental MedicineDongguk UniversityGyeongjuKorea
  5. 5.College of PharmacyCatholic University of DaeguGyeongsanKorea
  6. 6.College of Pharmacy and Research Institute of Pharmaceutical SciencesSeoul National UniversitySeoulKorea
  7. 7.Natural Products Research Institute, College of PharmacySeoul National UniversitySeoulKorea

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