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Archives of Pharmacal Research

, Volume 40, Issue 3, pp 364–372 | Cite as

Determination of urazamide in pharmaceutical preparation with room temperature ionic liquid

  • Narae Seo
  • Yoo Rim Lee
  • Hyun Sun Park
  • Quoc-Ky Truong
  • Jae-Yong Lee
  • Hyun Kyu Chung
  • Yusung Choi
  • Byeonghee Kim
  • Sang Beom Han
  • Kyeong Ho KimEmail author
Research Article

Abstract

A high performance liquid chromatographic method was developed and validated for the determination of urazamide in pharmaceutical preparation with novel green aqueous mobile phase modified with room temperature ionic liquids (RTILs). 1-Ethyl-3-methyl-imidazolium tetrafluoroborate ([EMIM][BF4]) was selected as a mobile phase additive to improve retention and avoid baseline disturbances at t0. Various mobile phase parameters such as cation moiety, chaotropic anion moiety, pH and concentration of RTILs were optimized to determine urazamide at the proper retention time. The assay was validated according to International Conference on Harmonization guidelines. The linearity of the calibration curve was good (r2 > 0.999). Intra-day precision varied between 0.50 and 1.23%. Relative standard deviations of inter-day precision ranged between 1.07 and 1.66%. Recoveries in tablets ranged between 99.7 and 101.2% and it was successfully applied to determine urazamide in pharmaceutical preparations.

Graphical Abstract

Keywords

Very polar compound Urazamide Room temperature ionic liquids (RTILs) Reverse phase high performance liquid chromatography (RP-HPLC) 

Notes

Acknowledgements

This research was supported by 2014 Research Grant from Kangwon National University (No. 120140173).

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Copyright information

© The Pharmaceutical Society of Korea 2017

Authors and Affiliations

  1. 1.College of PharmacyKangwon National UniversityChuncheonKorea
  2. 2.College of PharmacyChung-Ang UniversitySeoulKorea

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