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Archives of Pharmacal Research

, Volume 40, Issue 3, pp 328–337 | Cite as

Anti-inflammatory effects of secondary metabolites isolated from the marine-derived fungal strain Penicillium sp. SF-5629

  • Nguyen Thi Thanh Ngan
  • Tran Hong Quang
  • Kwan-Woo Kim
  • Hye Jin Kim
  • Jae Hak Sohn
  • Dae Gill Kang
  • Ho Sub Lee
  • Youn-Chul Kim
  • Hyuncheol OhEmail author
Research Article

Abstract

After the chemical investigation of the ethyl acetate extract of the marine-derived fungal strain Penicillium sp. SF-5629, the isolation and structural elucidation of eight secondary metabolites, including (3R,4S)-6,8-dihydroxy-3,4,7-trimethylisocoumarin (1), (3S,4S)-sclerotinin A (2), penicitrinone A (3), citrinin H1 (4), emodin (5), ω-hydroxyemodin (6), 8-hydroxy-6-methyl-9-oxo-9H-xanthene-1-carboxylate (7), and 3,8-dihydroxy-6-methyl-9-oxo-9H-xanthene-1-carboxylate (8) were carried out. Evaluation of the anti-inflammatory activity of these metabolites showed that 4 inhibited nitric oxide and prostaglandin E2 production in lipopolysaccharide-stimulated BV2 microglia, with IC50 values of 8.1 ± 1.9 and 8.0 ± 2.8 μM, respectively. The inhibitory function of 4 was confirmed based on decreases in inducible nitric oxide synthesis and cyclooxygenase-2 gene expression. In addition, 4 was found to suppress the phosphorylation of inhibitor kappa B-α, interrupt the nuclear translocation of nuclear factor kappa B, and decrease the activation of p38 mitogen-activated protein kinase.

Keywords

Marine-derived fungus Penicillium Anti-inflammatory Citrinin H1 

Notes

Acknowledgements

We acknowledge the financial support by grants from the Global R&D Center (GRDC, NRF-2010-00719) programs of the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT and Future Planning of Korea (MSIFP). This research was also supported by grants from the National Research Foundation of Korea (NRF) funded by the Korean Government (MSIP) (2008-0062484) (2015M3A9E3051054).

Compliance with ethical standards

Conflict of interest

All authors have no conflict of interest to declare.

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Copyright information

© The Pharmaceutical Society of Korea 2017

Authors and Affiliations

  • Nguyen Thi Thanh Ngan
    • 1
    • 2
  • Tran Hong Quang
    • 1
    • 3
  • Kwan-Woo Kim
    • 1
  • Hye Jin Kim
    • 1
  • Jae Hak Sohn
    • 4
  • Dae Gill Kang
    • 5
  • Ho Sub Lee
    • 5
  • Youn-Chul Kim
    • 1
    • 5
  • Hyuncheol Oh
    • 1
    • 5
    Email author
  1. 1.College of PharmacyWonkwang UniversityIksanRepublic of Korea
  2. 2.Institute of Genome ResearchVietnam Academy of Science and Technology (VAST)CaugiayVietnam
  3. 3.Institute of Marine BiochemistryVietnam Academy of Science and Technology (VAST)HanoiVietnam
  4. 4.College of Medical and Life SciencesSilla UniversityBusanRepublic of Korea
  5. 5.Hanbang Body-Fluid Research CenterWonkwang UniversityIksanRepublic of Korea

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