Annulohpoxylotol A and B, new sesquiterpenoids from the endophytic fungus Annulohypoxylon truncatum, are natural NF-κB inhibitors
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Two sesquiterpenoids, annulohpoxylotol A and B, were isolated along with five sterols from an ethyl acetate extract of cultures of the endophytic fungus Annulohypoxylon truncatum growing on leaves of Zizania caduciflora. The structures of the isolated compounds were established using one-dimensional (1D) and two-dimensional (2D)-NMR and mass spectrometry. The nuclear factor-kappa B (NF-κB) inhibitory activities of the isolated compounds stimulated with tumor necrosis factor-alpha (TNF-α) were measured using a luciferase reporter system. Annulohpoxylotol A (1) significantly inhibited NF-κB activation in a dose-dependent manner, with an IC50 of 7.11 μM, whereas annulohpoxylotol B (2) and ergone (7) moderately inhibited NF-κB transcriptional activity, with IC50 values of 19.24 and 17.51 μM, respectively.
KeywordsAnnulohypoxylon truncatum Sesquiterpenoid Endophytic fungus NF-κB
This research was supported by a Grant (K16281) awarded to the Korean Institute of Oriental Medicine by the Ministry of Education, Science and Technology (MEST); Priority Research Centers Program through the National Research Foundation (NRF) funded by the Ministry of Education, Science, and Technology (NRF-2016R1A6A1A03007648), and also supported by Basic Science Research Programs through NRF funded by the Ministry of Education (Grant No. NRF-2015R1D1A1A01057914).
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