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Archives of Pharmacal Research

, Volume 39, Issue 12, pp 1653–1662 | Cite as

Determination of piroxicam from rat articular tissue and plasma based on LC–MS/MS

  • Han Sol Kim
  • Ha Ra Cho
  • Myoung Jin Ho
  • Myung Joo Kang
  • Yong Seok ChoiEmail author
Research Article

Abstract

Osteoarthritis (OA) is the most common type of arthritis. To manage OA, in general, oral administration of non-steroidal anti-inflammatory drugs (NSAIDs) is used. Recently, the analgesic and anti-inflammatory efficacy of piroxicam (PX), a long-acting NSAID, by intra-articular (IA) administration in OA was reported, and the possibility that PX is distributed in articular tissues at a certain concentration was raised. Thus, herein, novel LC–MS/MS methods to detect PX in rat articular tissue and plasma are presented. For articular tissue, solvent extraction with acetonitrile for 12 h was employed and a protein precipitation method was used for the preparation of a plasma sample. The developed methods were validated by following the FDA guidelines, and the validated methods were successfully applied to a PK study of IA PX. The present study presents, to our knowledge, the first method of determining a drug in articular tissue. Additionally, the level of PX in articular tissue after IA PX administration was experimentally confirmed for the first time using the present methods. Therefore, the present methods provide a new direction for in vivo evaluation for IA PX formulations and contribute to the development of alternative IA PX formulations with better effects for the treatment of OA.

Keywords

Osteoarthritis Piroxicam Intra-articular administration Articular tissue Plasma LC–MS/MS 

Notes

Acknowledgments

We greatfully thank Prof. Young Wook Choi, Mr. Sung Rae Kim, Mr. Sang Hyun Kim (Chung-Ang University), and Ms. Sun Young Kim (Dankook University) for their technical supports. The present research was conducted by the research fund of Dankook University in 2014 (YSC).

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Copyright information

© The Pharmaceutical Society of Korea 2016

Authors and Affiliations

  • Han Sol Kim
    • 1
  • Ha Ra Cho
    • 1
  • Myoung Jin Ho
    • 1
  • Myung Joo Kang
    • 1
  • Yong Seok Choi
    • 1
    Email author
  1. 1.College of PharmacyDankook UniversityCheonanSouth Korea

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