Investigation of early and advanced stages in ovarian cancer using human plasma by differential scanning calorimetry and mass spectrometry
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Ovarian cancer is recognized with high mortality due to asymptomatic nature of the disease and difficulties in diagnosing early stage of the cancer. The present study evaluates the use of differential scanning calorimetry (DSC) in differentiating the severity of ovarian cancer from healthy women. 47 diseased women were subdivided into four stages with respect to clinical relevance and severity. Stages I–II were regarded as early stages and stages III–IV were regarded as advanced stages. The two average transition temperatures (T m ) increased with disease severity from 64.84 and 70.32 °C (healthy) to 68.46 and 75.24 °C (stage IV), respectively. T m were increased depending on clinical groups. In addition, the change in heat capacity was also dependent on the disease severity. To further support and investigate the nature of the proposed interactions, matrix assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) analysis is employed. The results suggest the differences in peptide expression between early and advanced stage of ovarian cancer, affected abundant proteins in plasma. The combined DSC and MS approach was supportive in identifying a unique signature of ovarian cancer stages, and demonstrates the potential of DSC as a complementary diagnostic tool in the evaluation of early stage ovarian cancer.
KeywordsDifferential scanning calorimetry Ovarian cancer Early stage Tm Diagnostic tool
This study was supported by a grant of the Korean Health Technology R&D Project, Ministry of Health & Welfare, Republic of Korea. (HI12C0050).
Compliance with ethical standards
Conflict of interest
The authors have declared that there is no conflict of interest.
- Chertov O, Biragyn A, Kwak LW, Simpson JT, Boronina T, Hoang VM, Prieto DA, Conrads TP, Veenstra TD, Fisher RJ (2004) Organic solvent extraction of proteins and peptides from serum as an effective sample preparation for detection and identification of biomarkers by mass spectrometry. Proteomics 4:1195–1203CrossRefPubMedGoogle Scholar
- Craig WY, Ledue TB, Ritchie RF (2001) Plasma proteins: clinical utility and interpretation. Foundation for Blood Research. http://www.fbr.org/Otherpages/TechnicalBrochures.aspx
- Ferencz A, Lőrinczy D, Fekecs T (2011) Differential scanning calorimetry, as a new method to monitor human plasma in melanoma patients with regional lymph node or distal metastases. In: Xi Y (ed) Skin cancer overview. InTech, Rijeka, pp 141–152Google Scholar
- Garbett NC, Merchant ML, Helm CW, Jenson AB, Klein JB, Chaires JB (2014) Detection of cervical cancer biomarker patterns in blood plasma and urine by differential scanning calorimetry and mass spectrometry. PLoS ONE 9:e84710. doi: 10.1371/journal.pone.0084710 CrossRefPubMedPubMedCentralGoogle Scholar