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Archives of Pharmacal Research

, Volume 39, Issue 2, pp 279–291 | Cite as

Hepatoprotective effects of zingerone on carbon tetrachloride- and dimethylnitrosamine-induced liver injuries in rats

  • Kyoung Ook Cheong
  • Dong-Su Shin
  • Jeonghyeon Bak
  • Changyong Lee
  • Kyung Wook Kim
  • Nam Kyung Je
  • Hae Young Chung
  • Sik Yoon
  • Jeon-Ok MoonEmail author
Research Article

Abstract

In this study, we investigated the hepatoprotective and anti-fibrotic effects of zingerone, one of the active components of ginger, against carbon tetrachloride (CCl4)- and dimethylnitrosamine (DMN)-induced liver injuries in rats, respectively. Oral administration of zingerone (10 mg/kg) reduced CCl4-induced abnormalities in liver histology, serum alanine aminotransferase and aspartate aminotransferase levels, and liver malondialdehyde levels. Zingerone treatment attenuated CCl4-induced increases in inflammatory mediators, including tumor necrosis factor-α, interleukin-1β, cyclooxygenase-2, and inducible nitric oxide synthase mRNA levels. Western blot analysis showed that zingerone suppressed activation of nuclear factor-kappa B (NF-κB) p65 and phosphorylation of extracellular signal-regulated kinase, c-Jun NH2-terminal kinase, and p38 mitogen-activated protein kinases (MAPKs). Liver fibrosis induced by DMN (10 mg/kg, intraperitoneally) was ameliorated by administration of zingerone (10 and 20 mg/kg, orally). Zingerone treatment reduced DMN-induced elevation of hydroxyproline content and hepatic stellate cell activation. In conclusion, zingerone showed antioxidative and anti-inflammatory effects in CCl4-intoxicated rats by inhibiting oxidative stress and NF-κB activation via blockade of the activation of upstream MAPKs. Moreover, zingerone had hepatoprotective and anti-fibrotic effects against DMN-induced liver injury suggesting its usefulness in the prevention of liver inflammation and the development of hepatic fibrosis.

Keywords

Zingerone Hepatoprotective effect Anti-fibrotic effect Antioxidant NF-κB MAPKs 

Notes

Acknowledgments

This work was supported by a 2-year Research Grant of Pusan National University. We thank the Aging Tissue Bank (Busan, Korea) for supplying research materials.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interests.

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Copyright information

© The Pharmaceutical Society of Korea 2015

Authors and Affiliations

  • Kyoung Ook Cheong
    • 1
  • Dong-Su Shin
    • 1
    • 3
  • Jeonghyeon Bak
    • 1
  • Changyong Lee
    • 1
  • Kyung Wook Kim
    • 1
  • Nam Kyung Je
    • 1
  • Hae Young Chung
    • 1
  • Sik Yoon
    • 2
  • Jeon-Ok Moon
    • 1
    Email author
  1. 1.College of PharmacyPusan National UniversityBusanKorea
  2. 2.Department of Anatomy College of MedicinePusan National UniversityYangsanKorea
  3. 3.Hazard Substances Analysis Team, Center for Food and Drug AnalysisBusan Regional Food & Drug AdministrationBusanKorea

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