Cytotoxic and anti-inflammatory activities of phenanthrenes from the medullae of Juncus effusus L.
- 390 Downloads
Bioactivity guided phytochemical investigation of the ethanol extract of the medullae of Juncus effusus resulted in the isolation of two new phenanthrenes, 8-hydroxymethyl-2-hydroxyl-1-methyl-5-vinyl-9,10-dihydrophenanthrene (1), and 5-(1-methoxyethyl)-1-methyl-phenanthren-2,7-diol (2) together with 15 known phenanthrenoids (3–17). The chemical structures of 1 and 2 were established by a combination of spectroscopic techniques. Compounds 1–15 and 17 were evaluated for their cytotoxic activities against five human cancer cell lines (SHSY-5Y, SMMC-7721, HepG-2, Hela and MCF-7) by CCK-8 assay, and their anti-inflammatory activities were also evaluated by inhibition on NO production in LPS-activated murine macrophage RAW 264.7 cells.
KeywordsJuncus effuses Juncaceae Phenanthrene Cytotoxicity Anti-inflammatory activity
This research work was supported financially by Collaborative Innovation Center of Modern Bio-manufacture, Anhui University (Y011008410). The authors are grateful to the staffs of the analytical group at the Modern Experiment Technology Center, Anhui University for measuring 400 MHz NMR and the State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences for measuring 600 MHz NMR and other spectral data.
Compliance with ethical standards
Conflict of interest
All authors declare that they have no conflicts of interest.