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Archives of Pharmacal Research

, Volume 39, Issue 2, pp 154–160 | Cite as

Cytotoxic and anti-inflammatory activities of phenanthrenes from the medullae of Juncus effusus L.

  • Wei Ma
  • Yue Zhang
  • Yun-Yun Ding
  • Feng Liu
  • Ning Li
Research Article

Abstract

Bioactivity guided phytochemical investigation of the ethanol extract of the medullae of Juncus effusus resulted in the isolation of two new phenanthrenes, 8-hydroxymethyl-2-hydroxyl-1-methyl-5-vinyl-9,10-dihydrophenanthrene (1), and 5-(1-methoxyethyl)-1-methyl-phenanthren-2,7-diol (2) together with 15 known phenanthrenoids (317). The chemical structures of 1 and 2 were established by a combination of spectroscopic techniques. Compounds 115 and 17 were evaluated for their cytotoxic activities against five human cancer cell lines (SHSY-5Y, SMMC-7721, HepG-2, Hela and MCF-7) by CCK-8 assay, and their anti-inflammatory activities were also evaluated by inhibition on NO production in LPS-activated murine macrophage RAW 264.7 cells.

Keywords

Juncus effuses Juncaceae Phenanthrene Cytotoxicity Anti-inflammatory activity 

Notes

Acknowledgments

This research work was supported financially by Collaborative Innovation Center of Modern Bio-manufacture, Anhui University (Y011008410). The authors are grateful to the staffs of the analytical group at the Modern Experiment Technology Center, Anhui University for measuring 400 MHz NMR and the State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences for measuring 600 MHz NMR and other spectral data.

Compliance with ethical standards

Conflict of interest

All authors declare that they have no conflicts of interest.

Supplementary material

12272_2015_680_MOESM1_ESM.docx (2.2 mb)
Supplementary material 1 (DOCX 2296 kb)

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Copyright information

© The Pharmaceutical Society of Korea 2015

Authors and Affiliations

  • Wei Ma
    • 1
  • Yue Zhang
    • 1
  • Yun-Yun Ding
    • 1
  • Feng Liu
    • 1
  • Ning Li
    • 1
  1. 1.School of PharmacyAnhui Medical UniversityHefeiChina

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