Archives of Pharmacal Research

, Volume 38, Issue 12, pp 2208–2215 | Cite as

Enzymatic synthesis of novel isobavachalcone glucosides via a UDP-glycosyltransferase

  • Hong-Mei Li
  • Jae Kyoung Lee
  • Li-Juan Nie
  • Qiang Huo
  • Tao Ma
  • Jae Kyung Sohng
  • Young-Soo Hong
  • Cheng-Zhu Wu
Research Article

Abstract

Glycosylation is often used to improve a natural product’s properties such as water solubility, chemical stability, pharmacological potency, and structure diversification. In this study, we studied the enzymatic synthesis of novel isobavachalcone glucosides using a UDP-glycosyltransferase (YjiC) from Bacillus licheniformis DSM-13. The chemical structures of compounds 1 and 2 were elucidated by spectroscopic techniques, including LC–MS, MS, and NMR. Meanwhile, the parameters of glycosylation reaction such as incubation time, UDP-glucose concentration, and pH of buffer were also optimized during this study. Furthermore, the compounds 1 and 2 exhibited weak anti-proliferative activities against five human cancer cell lines, with IC50 values ranging from 58.6 to 86.6 μM.

Keywords

Isobavachalcone Glycosylation YjiC Diversification Cytotoxicity 

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Copyright information

© The Pharmaceutical Society of Korea 2015

Authors and Affiliations

  • Hong-Mei Li
    • 1
  • Jae Kyoung Lee
    • 2
  • Li-Juan Nie
    • 1
  • Qiang Huo
    • 1
  • Tao Ma
    • 1
  • Jae Kyung Sohng
    • 3
  • Young-Soo Hong
    • 2
  • Cheng-Zhu Wu
    • 1
  1. 1.Faculty of PharmacyBengbu Medical CollegeBengbuChina
  2. 2.Chemical Biology Research Center, KRIBBChungbukKorea
  3. 3.Department of Pharmaceutical Engineering, Institute of Biomolecule ReconstructionSun Moon UniversityAsanKorea

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