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Archives of Pharmacal Research

, Volume 38, Issue 5, pp 620–627 | Cite as

Suppressive effects of coixol, glyceryl trilinoleate and natural products derived from Coix Lachryma-Jobi var. ma-yuen on gene expression, production and secretion of airway MUC5AC mucin

  • Hyun Jae Lee
  • Jiho Ryu
  • Su Hyun Park
  • Eun-Kyoung Seo
  • Ah-Reum Han
  • Sang Kook Lee
  • Yeong Shik Kim
  • Jang-Hee Hong
  • Jeong Ho Seok
  • Choong Jae LeeEmail author
Research Article

Abstract

In this study, we investigated whether natural products including coixol derived from Coix Lachryma-Jobi var. ma-yuen affect MUC5AC mucin gene expression, production and secretion from airway epithelial cells. Confluent NCI-H292 cells were pretreated with oleic acid, linoleic acid, glyceryl trilinoleate, beta-stigmasterol or coixol for 30 min and then stimulated with PMA (phorbol 12-myristate 13-acetate), EGF (epidermal growth factor) or TNF-α (tumor necrosis factor-α) for 24 h. The MUC5AC mucin gene expression, mucin protein production and secretion were measured by RT-PCR and ELISA. The results were as follows: (1) Oleic acid, linoleic acid, glyceryl trilinoleate, beta-stigmasterol and coixol inhibited the expression of MUC5AC mucin gene induced by PMA from NCI-H292 cells; (2) Oleic acid, linoleic acid, glyceryl trilinoleate, beta-stigmasterol and coixol also inhibited the production of MUC5AC mucin protein induced by the same inducers from NCI-H292 cells; (3) Coixol inhibited the expression of MUC5AC mucin gene and production of MUC5AC mucin protein, induced by EGF or TNF-α from NCI-H292 cells; (4) Coixol decreased PMA-induced MUC5AC mucin secretion from NCI-H292 cells. This result suggests that coixol, the characteristic component among the examined five natural products derived from C. Lachryma-Jobi var. ma-yuen, can regulate gene expression, production and secretion of mucin, by directly acting on airway epithelial cells.

Keywords

Airway mucin MUC5AC Coixol CoixLachryma-Jobi var. ma-yuen 

Abbreviations

EGF

Epidermal growth factor

PMA

Phorbol 12-myristate 13-acetate

TNF-α

Tumor necrosis factor-α

PBS

Phosphate-buffered saline

RT-PCR

Reverse transcription-polymerase chain reaction

ELISA

Enzyme-linked immunosorbent assay

Notes

Acknowledgments

This research was supported by a grant (12172KFDA989) from Korea Food & Drug Administration in 2012.

Conflict of interest

The authors have declared that there are no conflicts of interest.

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Copyright information

© The Pharmaceutical Society of Korea 2014

Authors and Affiliations

  • Hyun Jae Lee
    • 1
  • Jiho Ryu
    • 1
  • Su Hyun Park
    • 1
  • Eun-Kyoung Seo
    • 2
  • Ah-Reum Han
    • 2
  • Sang Kook Lee
    • 3
  • Yeong Shik Kim
    • 3
  • Jang-Hee Hong
    • 1
  • Jeong Ho Seok
    • 1
  • Choong Jae Lee
    • 1
    Email author
  1. 1.Department of Pharmacology, School of MedicineChungnam National UniversityDaejeonKorea
  2. 2.Department of Pharmacy, College of PharmacyEwha Womans UniversitySeoulKorea
  3. 3.Department of Pharmacy, College of PharmacySeoul National UniversitySeoulKorea

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