Archives of Pharmacal Research

, Volume 37, Issue 6, pp 773–782

Platycodi radix saponin inhibits α-glucosidase in vitro and modulates hepatic glucose-regulating enzyme activities in C57BL/KsJ-db/db mice

  • Jeom-Sook Lee
  • Myung-Sook Choi
  • Kown-Il Seo
  • Jin Lee
  • Hae-In Lee
  • Ju-Hye Lee
  • Myung-Joo Kim
  • Mi-Kyung Lee
Research Article

DOI: 10.1007/s12272-013-0259-3

Cite this article as:
Lee, JS., Choi, MS., Seo, KI. et al. Arch. Pharm. Res. (2014) 37: 773. doi:10.1007/s12272-013-0259-3

Abstract

This study investigated anti-diabetic activity of a concentrated saponin fraction from Platycodi radix (SK1) in C57BL/KsJ-db/db mice and its underlying mechanism. Mice were fed diet with 0.5 % SK1 (w/w) for 6 weeks. SK1 significantly lowered the blood glucose and glycosylated hemoglobin levels and improved glucose and insulin tolerance. The plasma and pancreatic insulin and C-peptide levels and fecal cholesterol content were increased, whereas plasma urea nitrogen, free fatty acid and triglyceride levels were decreased by SK1 supplementation. Glucokinase (GK) activity in the liver was significantly higher in the SK1 group than the control group, whereas the glucose-6-phosphatase (G6Pase) activity was lower. SK1 significantly down-regulated GK mRNA expression compared to the control group but did not affect G6Pase and glucose transporter 2 mRNA. Phosphoenolpyruvate carboxykinase activity and mRNA levels did not differ between groups. SK1 also markedly inhibited the small intestinal disaccharidases activities compared to those of control db/db mice. Furthermore, SK1 was a more effective α-glucosidase inhibitor than acarbose in vitro. Overall, these findings suggest that SK1 is a potential glucose-lowering agent that functions via inhibition of carbohydrate digestive enzyme activities and modulation of glucose-regulating enzyme activities in db/db mice.

Keywords

α-Glucosidase inhibitor Glucose metabolism Liver Platycodi radix saponin Type 2 diabetes 

Copyright information

© The Pharmaceutical Society of Korea 2013

Authors and Affiliations

  • Jeom-Sook Lee
    • 1
  • Myung-Sook Choi
    • 2
  • Kown-Il Seo
    • 1
  • Jin Lee
    • 1
  • Hae-In Lee
    • 1
  • Ju-Hye Lee
    • 1
    • 3
  • Myung-Joo Kim
    • 4
  • Mi-Kyung Lee
    • 1
  1. 1.Department of Food and NutritionSunchon National UniversityJeonnamKorea
  2. 2.Department of Food Science and NutritionKyungpook National UniversityDaeguKorea
  3. 3.Research Institute of Basic ScienceSunchon National UniversitySuncheonKorea
  4. 4.Department of Hotel CuisineSuseong CollegeDaeguKorea

Personalised recommendations