Archives of Pharmacal Research

, Volume 36, Issue 7, pp 912–917

Antioxidant effect of astragalin isolated from the leaves of Morus alba L. against free radical-induced oxidative hemolysis of human red blood cells

  • Jiwon Choi
  • Hyun Ju Kang
  • Sung Zoo Kim
  • Tae Oh Kwon
  • Seung-Il Jeong
  • Seon Il Jang
Research Article


We evaluated the antioxidant properties of mulberry leaves extract (MLE) and flavonoids isolated from MLE. MLE was prepared by extraction with methanol. Flavonoids were analyzed by high-performance liquid chromatography. Oxidative hemolysis of normal human red blood cells (RBCs) was induced by the aqueous peroxyl radical [2,2′-Azobis (2-amidinopropane) dihydrochloride, AAPH]. MLE contained three flavonoids in the order quercetin (QC) > kaempferol (KF) > astragalin (AG). Oxidative hemolysis of RBCs induced by AAPH was suppressed by MLE, AG, KF, and QC in a time- and dose-dependent manner. MLE and these three flavonoids prevented the depletion of cystosolic antioxidant glutathione (GSH) in RBCs. AG had the greatest protective effect against AAPH-induced oxidative hemolysis and GSH depletion in RBCs.


Antioxidant activity Astragalin Flavonoid Mulberry leaves Oxidative damage Red blood cells 

Supplementary material

12272_2013_90_MOESM1_ESM.pdf (40 kb)
Supplementary material 1 (PDF 40 kb)


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Copyright information

© The Pharmaceutical Society of Korea 2013

Authors and Affiliations

  • Jiwon Choi
    • 1
    • 3
  • Hyun Ju Kang
    • 2
  • Sung Zoo Kim
    • 3
  • Tae Oh Kwon
    • 4
  • Seung-Il Jeong
    • 5
  • Seon Il Jang
    • 2
  1. 1.Department of Radiological SciencesJeonju UniversityJeonjuKorea
  2. 2.Department of Healthcare & ScienceJeonju UniversityJeonjuKorea
  3. 3.Department of Physiology, School of MedicineChonbuk National UniversityJeonjuKorea
  4. 4.College of Life Science and Natural ResourcesWonkwang UniversityIksanKorea
  5. 5.Jeonju Biomaterials InstituteJeonjuKorea

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