Effects of the rhizomes of Atractylodes japonica and atractylenolide I on allergic response and experimental atopic dermatitis
- 356 Downloads
Although some anti-allergic activities of the rhizome of Atractylodes japonica have been previously reported, the active principle(s) for anti-allergic action is not fully elucidated and the effect of this plant material on atopic dermatitis (AD) is not known. In this study, the 70% ethanol extract of the rhizome of A. japonica was found to significantly inhibit 5-lipoxygenase (5-LOX)-catalyzed leukotrienes (LT) production from rat basophilic leukemia (RBL)-1 cells. From the extract of A. japonica, three major sesquiterpene derivatives including atractylenolide I, atractylenolide III and eudesma-4,7-dien-8-one were successfully isolated. Among these compounds, only atractylenolide I was shown to strongly inhibit 5-LOX from RBL-1 cells (IC50 = 18.6 μM). To evaluate the effects of experimental AD, the ethanol extract of A. japonica (200 mg/day) was administered orally to hapten-treated NC/Nga mice which is an animal model of AD. It was firstly found that the extract significantly inhibited AD-like symptoms in mice, as judged by severity score and scratching behavior. Taken together, it is concluded that A. japonica possesses the inhibitory activity on 5-LOX and an animal model of AD, and atractylenolide I may contribute, at least in part, to these anti-allergic actions of A. japonica.
Key wordsAtractylodes japonica Atractylenolide I 5-Lipoxygenase Anti-allergy Atopic dermatitis
Unable to display preview. Download preview PDF.
- Choi, K. H., Jeong, S. I., Lee, J. H., Hwang, B. S., Lee, S., Choi, B. K., and Jung, K. Y., Acetylene compound isolated from Atractylodes japonica stimulates the contractility of rat distal colon via inhibiting the nitrergic-purinergic relaxation. J. Ethnopharmacol., 134, 104–110 (2011b).PubMedCrossRefGoogle Scholar
- Ding, H.-Y., Wu, Y.-C., and Lin, H.-C., Phytochemical and pharmacological studies on Chinese changzhu. J. Chin. Chem. Soc., 47, 561–566 (2000).Google Scholar
- Han, S. B., Lee, C. W., Yoon, Y. D., Lee, J. H., Kang, J. S., Lee, K. H., Yoon, W. K., Lee, K., Park, S. K., and Kim, H. M., Prevention of arthritic inflammation using an oriental herbal combination BDX-1 isolated from Achyranthes bidentata and Atractylodes japonica. Arch. Pharm. Res., 28, 902–908 (2005).PubMedCrossRefGoogle Scholar
- Hong, M. H., Kim, J.-H., Bae, H., Lee, N.-Y., Shin, Y.-C., Kim, S.-H., and Ko, S.-G., Atractylodes japonica Koidzumi inhibits the production of pro-inflammatory cytokines through inhibition of the NF-κB/IκB signal pathway in HMC-1 human mast cells. Arch. Pharm. Res., 33, 843–851 (2010).PubMedCrossRefGoogle Scholar
- Jang, M. H., Shin, M. C., Kim, Y. J., Kim, C. J., Kim., Y., and Kim, E. H., Atractylodes japonica suppresses lipopolysaccharide-stimulated expressions of inducible nitric oxide synthase and cyclooxygenase-2 in RAW 264.7 macrophages. Biol. Pharm. Bull., 27, 324–327 (2004).PubMedCrossRefGoogle Scholar
- Jeong, S.-I., Kim, S.-Y., Kim, S.-J., Hwang, B.-S., Kwon, T.-H., Yu, K.-Y., Hang, S.-H., Suzuki, K., and Kim, K.-J., Antibacterial activity of phytochemicals isolated from Atractylodes japonica against methicillin-resistant Staphylococcus aureus. Molecules, 15, 7395–7402 (2010).PubMedCrossRefGoogle Scholar
- Pu, S., Pu, H., Jin, D., Qu, X., Zhang, T., Jiang, G., Bai, Y., and Jin, H., Effect of n-butanol extract of Japanese atractylodes (Atractylodes japonica) on gastric ulcer in rats. Zhongcaoyao, 27, 410–413 (1996).Google Scholar
- Yamamoto, K., Yamashita, K., Hitomi, N., Suzuki, A., and Yoneda, K., Studies on the constituents of atractylodes rhizome, constituents in the rhizome of Atractylodes japonica and TLC analysis of Jutsu. Shoyakugaku Zasshi, 47, 12–16 (1993).Google Scholar