Archives of Pharmacal Research

, Volume 35, Issue 4, pp 723–732 | Cite as

Anti-inflammatory effects of (Z)-ligustilide through suppression of mitogen-activated protein kinases and nuclear factor-κB activation pathways

  • Ji Won Chung
  • Ran Joo Choi
  • Eun-Kyoung Seo
  • Joo-Won Nam
  • Mi-Sook Dong
  • Eun Myoung Shin
  • Lian Yu Guo
  • Yeong Shik KimEmail author
Research Articles Drug Actions


The roots of Angelica tenuissima have been commonly used for the treatment of cardiovascular diseases and menstrual discomfort in Asian countries, such as China and Korea. The primary volatile flavor components are essential oil ingredients, phthalide lactones. In this study, (Z)-ligustilide was tested for its anti-inflammatory activities in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages. We found that (Z)-ligustilide strongly inhibitis the induction of LPS-induced inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) at both the mRNA and protein levels in a dose-dependent manner. The transcriptional activity of nuclear factor kappa B (NF-B) was also down-regulated in a concentration-dependent manner. Further study revealed that (Z)-ligustilide inhibited the phosphorylation and subsequent degradation of IBα, an inhibitor protein of NF-B. In addition, (Z)-ligustilide inhibited the phosphorylation of p38 mitogen-activated protein kinase (p38 MAPK), extracellular signal-regulated kinase (ERK) and c-Jun NH2-terminal kinase (JNK) in a dose-dependent manner. Taken together, these data suggest that (Z)-ligustilide can exert its antiinflammatory effects by regulating the NF-B and MAPK signal pathways.

Key words

(Z)-Ligustilide Anti-inflammation Nuclear factor-κB Mitogen-activated protein kinases 


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Copyright information

© The Pharmaceutical Society of Korea and Springer Netherlands 2012

Authors and Affiliations

  • Ji Won Chung
    • 1
  • Ran Joo Choi
    • 1
  • Eun-Kyoung Seo
    • 2
  • Joo-Won Nam
    • 2
  • Mi-Sook Dong
    • 3
  • Eun Myoung Shin
    • 1
  • Lian Yu Guo
    • 1
  • Yeong Shik Kim
    • 1
    Email author
  1. 1.Natural Products Research Institute, College of PharmacySeoul National UniversitySeoulKorea
  2. 2.College of Pharmacy and the Center for Cell Signaling & Drug Discovery ResearchEwha Woman’s UniversitySeoulKorea
  3. 3.School of Life Science & BiotechnologyKorea UniversitySeoulKorea

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