Comparative evaluation of the antioxidant effects of the natural vitamin C analog 2-O-β-D-glucopyranosyl-L-ascorbic acid isolated from Goji berry fruit
- 826 Downloads
2-O-β-D-Glucopyranosyl-L-ascorbic acid (AA-2βG) is a natural derivative of vitamin C (Lascorbic acid, AA) isolated from Goji berry (Lycium barbarum L.) fruit. We evaluated the antioxidant activities of AA-2βG and AA using in vitro and in vivo model systems. In vitro radical scavenging assays demonstrated that AA-βG was capable of scavenging 1,1-diphenyl-2-picryl-hydrazyl and hydroxyl peroxide and inhibiting H2O2-induced hemolysis better than AA. AA-2βG and AA had similar hydroxyl radical scavenging capabilities, but AA-2βG was incapable of scavenging superoxide anion radicals, and its capacity to scavenge nitrite (NO2 −) was lower than that of AA. The overall in vitro reduction capability of AA-2βG was also significantly lower than that of AA. Moreover, in vivo studies demonstrated that AA-2βG was capable of protecting the liver against carbon tetrachloride-induced acute liver injury in mice. These results suggest that AA-2βG is an important antioxidant component of Goji berry fruit, which may share similar but distinct antioxidant mechanistic properties with AA. This study furthers our understanding of the mechanisms of Goji berry fruit pharmacological activities on antiaging and antitumor properties as a traditional medicine and dietary supplement.
Key wordsAA-2βG Vitamin C Analog Antioxidant Lycium barbarum L.
Unable to display preview. Download preview PDF.
- Chen, Q., Espey, M. G., Sun, A. Y., Pooput, C., Kirk, K. L., Krishna, M. C., Khosh, D. B., Drisko, J., and Levine, M., Pharmacologic doses of ascorbate act as a prooxidant and decrease growth of aggressive tumor xenografts in mice. Proc. Natl. Acad. Sci. U. S. A., 105, 11105–11109 (2008).PubMedCrossRefGoogle Scholar
- Kim, S. K., Kim, H. J., Choi, S. E., Park, K. H., Choi, H. K., and Lee, M. W. Anti-oxidative and inhibitory activities on nitric oxide (NO) and prostaglandin E2 (COX-2) production of flavonoids from seeds of Prunus tomentosa Thunberg. Arch. Pharm. Res., 31, 424–428 (2008).PubMedCrossRefGoogle Scholar
- Park, J. K., Jeong, D. H., Park, H. Y., Son, K. H., Shin, D. H., Do, S. H., Yang, H. J., Yuan, D. W., Hong, I. H., Goo, M. J., Lee, H. R., Ki, M. R., Ishigami, A., and Jeong, K. S., Hepatoprotective effect of Arazyme on CCl4-induced acute hepatic injury in SMP30 knock-out mice. Toxicology, 246, 132–142 (2008).PubMedCrossRefGoogle Scholar
- Toyada-Ono, Y., Maeda, M., Nakao, M., Yoshimura, M., Sugiura-Tomimori, N., Fukami, H., Nishioka, H., Miyashita, Y., and Kojo, S., A novel vitamin C analog, 2-O-(beta-D-Glucopyranosyl)ascorbic acid: examination of enzymatic synthesis and biological activity. J. Biosci. Bioeng., 99, 361–365 (2005).PubMedCrossRefGoogle Scholar
- Zhang, Z., Liu, X., Wu, T., Liu, J., Zhang, X., Yang, X., Goodheart, M. J., Engelhardt, J. F., and Wang, Y., Selective suppression of cervical cancer HeLa cells by 2-O-β-D-glucopyranosyl-L-ascorbic acid isolated from the fruit of Lycium barbarum L. Cell Biol. Toxicol., 27, 107–121 (2011).PubMedCrossRefGoogle Scholar