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Polyozellin blocks tumor necrosis factor α-induced interleukin 8 and matrix metalloproteinase 7 production in the human intestinal epithelial cell line HT-29

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Abstract

Polyozellin isolated from Polyozellus multiplex (Thelephoraceae) displays potent anti-inflammatory effects in murine macrophages. Here we evaluated whether polyozellin has the potential to ameliorate diseases characterized by mucosal inflammation in intestinal epithelial HT-29 cells. Polyozellin significantly inhibited tumor necrosis factor (TNF)-α-induced interleukin-8 secretion and mRNA expression. Moreover, polyozellin suppressed the expression of matrix metalloproteinase-7 mRNA and extracellular pro-matrix metalloproteinase-7 secretion. A signal transduction study revealed that polyozellin significantly attenuates TNF-α-mediated p38 phosphorylation, inhibitory factor κBα degradation, and nuclear factor-κB-mediated transcriptional activation. Collectively, these results suggest that polyozellin has the potential to attenuate intestinal inflammation and shed light on the novel signal pathway evoked by TNF-α during intestinal inflammation.

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Authors and Affiliations

  1. Institute of Pharmaceutical Research and Development, College of Pharmacy, Wonkwang University, Iksan, Chonbuk, 570-749, Korea

    Sung Hee Lee & Dong Hwan Sohn

  2. School of Applied Biosciences, College of Agriculture and Life Sciences, Kyungpook National University, Daegu, 702-701, Korea

    Kyung-Sik Song

  3. Digestive Disease Research Institute, Wonkwang University School of Medicine, Iksan, 570-749, Korea

    Geom Seog Seo

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  1. Sung Hee Lee
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  2. Kyung-Sik Song
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  3. Dong Hwan Sohn
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  4. Geom Seog Seo
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Correspondence to Geom Seog Seo.

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Lee, S.H., Song, KS., Sohn, D.H. et al. Polyozellin blocks tumor necrosis factor α-induced interleukin 8 and matrix metalloproteinase 7 production in the human intestinal epithelial cell line HT-29. Arch. Pharm. Res. 34, 91–97 (2011). https://doi.org/10.1007/s12272-011-0111-6

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  • DOI: https://doi.org/10.1007/s12272-011-0111-6

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