Archives of Pharmacal Research

, Volume 33, Issue 9, pp 1307–1315 | Cite as

Inhibition of DNA topoisomerases I and II and cytotoxicity of compounds from Ulmus davidiana var. japonica

  • Ming Shan Zheng
  • Yeun-Kyung Lee
  • Ying Li
  • Kyoung Hwangbo
  • Chong-Soon Lee
  • Jae-Ryong Kim
  • Sunny Kyung-Seon Lee
  • Hyun-Wook Chang
  • Jong-Keun Son
Research Articles Drug Discovery and Development

Abstract

Twenty five compounds including ten triterpenes (1–3, 5–11), six flavonoids (12–15, 24, 25), five lignans (17, 18, 21–23), two butenyl clohexnone glycosides (19–20), one fructofuranoside (16) and one fatty acid (4) were isolated from the roots of Ulmus davidiana var. japonica. The structures of those compounds were identified by comparing their physicochemical and spectral data with those of published in literatures. All the compounds were evaluated for DNA topoisomerase inhibitory activities and cytotoxicities. Among the purified compounds, 4 and 19 showed more potent inhibitory acitivities (IC50: 39 and 19 μM, respectively) than camptothecin, as the positive control (IC50: 46 μM) against topoisomerase I. Compounds, 4, 10, 12, 19, 24 and 25 showed strong inhibitory activities toward DNA topoisomerase II (IC50: 0.1, 0.52, 0.47, 0.42, 0.17 μM and 17 nM, respectively), which were more potent than that of etoposide as positive control (IC50: 20 μM). In A549 cell line, 5 and 6 showed cytotoxicities (IC50: 4 μM and 3 μM, respectively, with IC50 of camptothecin as positive control: 10.3 μM). In the HepG2 cell line, 3, 5 and 7 showed cytotoxicity (IC50: 4, 3 and 4 μM, respectively, with IC50 of camptothecin: 0.3 μM). Compounds 6, 12 and 23 showed cytotoxicities in the HT-29 cell line (IC50: 19, 19 and 15 μM, respectively, with IC50 of camptothecin: 2 μM).

Key words

Ulmus davidiana var. japonica Topoisomerase Cytotoxicity 

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Copyright information

© The Pharmaceutical Society of Korea and Springer Netherlands 2010

Authors and Affiliations

  • Ming Shan Zheng
    • 1
    • 2
  • Yeun-Kyung Lee
    • 1
  • Ying Li
    • 1
  • Kyoung Hwangbo
    • 1
  • Chong-Soon Lee
    • 3
  • Jae-Ryong Kim
    • 4
  • Sunny Kyung-Seon Lee
    • 5
  • Hyun-Wook Chang
    • 1
  • Jong-Keun Son
    • 1
  1. 1.College of PharmacyYeungnam UniversityGyongsanKorea
  2. 2.Yanbian UniversityYanjiChina
  3. 3.Department of Biochemistry, College of ScienceYeungnam UniversityGyongsanKorea
  4. 4.Department of Biochemistry and Molecular Biology, Aging-associated Vascular Disease Research Center, College of MedicineYeungnam UniversityDaeguKorea
  5. 5.Department of Pathology and Laboratory MedicineUniversity of California DavisSacramentoUSA

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