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Archives of Pharmacal Research

, Volume 32, Issue 6, pp 813–822 | Cite as

Inhibitory effect of saponin fraction from Codonopsis lanceolata on immune cell-mediated inflammatory responses

  • Se Eun Byeon
  • Wahn Soo Choi
  • Eock Kee Hong
  • Jaehwi Lee
  • Man Hee Rhee
  • Hwa-Jin Park
  • Jae Youl Cho
Research Articles Drug Discovery and Development

Abstract

Saponin components are known to be pharmaceutically, cosmetically and nutraceutically valuable principles found in various herbal medicine. In this study, we evaluated the inhibitory role of saponin fraction (SF), prepared from C. lanceolata, an ethnopharmacologically famous plant, on various inflammatory responses managed by monocytes, macrophages, lymphocytes and mast cells. SF clearly suppressed the release of nitric oxide (NO) and tumor necrosis factor (TNF)-α, but not prostaglandin E2 (PGE2). While this fraction did not scavenge the reactivity of SNP-induced radicals in RAW264. 7 cells, it negatively modulated the phagocytic uptake of macrophages treated with FITC-dextran. Interestingly, SF completely diminished cell-cell adhesion events induced by both CD29 and CD43, but not cell-fibronectin adhesion. Concanavalin (Con) A [as well phytohemaglutinin A (PHA)]-induced proliferation of splenic lymphocytes as well as interferon (IFN)-γ production were also clearly suppressed by SF treatment. Finally, SF also significantly blocked the degranulation process of mast cell line RBL-2H3 cell as assessed by DNP-BSA-induced β-hexosaminidase activity. The anti-inflammatory activities of SF on NO production seemed to be due to inhibition of nuclear factor (NF)-κB activation signaling, since it blocked the phosphorylation of inhibitor of κB (IκB)α as well as inducible NO synthase (iNOS) expression. Therefore, these results suggest that SF may be considered as a promising herbal medicine with potent anti-inflammatory actions.

Key words

Codonopsis lanceolata Saponin fraction Anti-inflammatory effects Inflammatory responses Inflammatory cells 

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Copyright information

© The Pharmaceutical Society of Korea 2009

Authors and Affiliations

  • Se Eun Byeon
    • 1
  • Wahn Soo Choi
    • 2
  • Eock Kee Hong
    • 3
  • Jaehwi Lee
    • 4
  • Man Hee Rhee
    • 5
  • Hwa-Jin Park
    • 6
  • Jae Youl Cho
    • 1
  1. 1.School of Bioscience and Biotechnology, and Institute of Bioscience and BiotechnologyKangwon National UniversityChuncheonKorea
  2. 2.College of MedicineKunkuk UniversityChungjuKorea
  3. 3.Department of Bioengineering and TechnologyKangwon National UniversityChuncheonKorea
  4. 4.College of PharmacyChung-Ang UniversitySeoulKorea
  5. 5.Laboratory of Veterinary Physiology & Signaling, College of Veterinary MedicineKyungpook National UniversityDaeguKorea
  6. 6.Department of Biomedical Laboratory Science, College of Biomedical Science and Engineering, and Regional Research CenterInje UniversityGimhaeKorea

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