Archives of Pharmacal Research

, Volume 31, Issue 1, pp 83–87 | Cite as

trans-resveratrol relaxes the corpus cavernosum ex vivo and enhances testosterone levels and sperm quality in vivo

  • Sunhee Shin
  • Jeong Hee Jeon
  • Dongsun Park
  • Min-Jung Jang
  • Jae Hong Choi
  • Bong-Ho Choi
  • Seong Soo Joo
  • Sang-Seop Nahm
  • Jong-Choon Kim
  • Yun-Bae Kim
Article

Abstract

We examined the effects of trans-resveratrol on male reproductive functions; ex-vivo penile erection and in-vivo sperm counts and quality. For the ex-vivo study, the relaxation effects of resveratrol on isolated New Zealand white rabbit corpus cavernosum, precontracted by phenylephrine (5×10−5 M) were measured. The in-vivo study measured reproductive organ weights, blood testosterone levels, testicular histopathology, sperm counts, as well as the epididymal sperm motility and deformity of male ICR mice given an oral dose of resveratrol (50 mg/kg) for 28 days. Resveratrol elicited a concentration-dependent relaxing effect on corpus cavernosum, leading to a median effective concentration (EC50) of 0.29 mg/mL. Repeated treatment with resveratrol (50 mg/kg) did not cause an increase in body weight, reproductive organ weight or testicular microscopic findings; however, resveratrol did elicit an increase in blood testosterone concentration, testicular sperm counts and epididymal sperm motility by 51.6%, 15.8% and 23.3%, respectively, without influence on sperm deformity. In conclusion, we propose that resveratrol has a positive effect on male reproductive function by triggering a penile erection, as well as enhancing blood testosterone levels, testicular sperm counts, and epididymal sperm motility.

Key words

Resveratrol Corpus cavernosum relaxation Testosterone Sperm counts and motility 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Akmal, M., Qadri, J. Q., Al-Waili, N. S., Thangal, S., Haq, A., and Saloom, K. Y., Improvement in human semen quality after oral supplementation of vitamin C. J. Med. Food, 9, 440–442 (2006).PubMedCrossRefGoogle Scholar
  2. Andersson, K. E. and Wagner, G, Physiology of penile erection. Physiol. Rev., 75, 191–236 (1995).PubMedGoogle Scholar
  3. Carreau, S., Silandre, D., Bourguiba, S., Hamden, K., Said, L., Lambard, S., Galeraud-Denis, I., and Delalande, C., Estrogens and male reproduction: a new concept. Braz. J. Med. Biol. Res. 40, 761–768 (2007).PubMedCrossRefGoogle Scholar
  4. Chen, C. K. and Pace-Asciak, C. R., Vasorelaxing activity of resveratrol and quercetin in isolated rat aorta. Gen. Pharmac., 27, 363–366 (1996).Google Scholar
  5. Crowell, J. A., Korytko, P. J., Morrissey, R. L., Booth, T. D., and Levine, B. S., Resveratrol-associated renal toxicity. Toxicol. Sci., 82, 614–619 (2004).PubMedCrossRefGoogle Scholar
  6. Fouad, R., Vivien, K., and Ronald, S., Male sexual function and its disorders: physiology, pathophysiology, clinical investigation, and treatment. Endocr. Rev., 22, 342–388 (1993).Google Scholar
  7. Gehm, B. D., McAndrews, J. M., Chien, P. Y., and Jameson, J. L., Resveratrol, a polyphenolic compound found in grapes and wine, is a agonist for the estrogen receptor. Proc. Natl. Acad. Sci. USA, 94, 14138–14143 (1997).PubMedCrossRefGoogle Scholar
  8. Gruenewald, D. A. and Matsumoto, A. M., Testosterone supplementation therapy for older men: potential benefits and risks. J. Am. Geriatr. Soc., 51, 101–115 (2003).PubMedCrossRefGoogle Scholar
  9. Henry, L. A. and Witt, D. M., Resveratrol: phytoestrogen effects on reproductive physiology and behavior in female rats. Horm. Behav., 41, 220–228 (2002).PubMedCrossRefGoogle Scholar
  10. Hinrichsen, M. J. and Blagaier, J. A., Evidence supporting the existence of sperm maturation in the human epididymis. J. Reprod. Fertil., 60, 291–294 (1980).PubMedCrossRefGoogle Scholar
  11. Holdcraft, R. W. and Braun, R. E., Hormonal regulation of spermatogenesis. Int. J. Androl., 27, 335–342 (2004).PubMedCrossRefGoogle Scholar
  12. Jeon, J. H., Shin, S., Park, D., Jang, J. Y., Choi, B., Kang, J.-K., Hwang, S.-Y., and Kim, J.-C., Kim, B.-Y., Kim, M. R., and Kim, Y.-B., Fermentation filtrates of Rubus coreanus relax the corpus cavernosum and increase the sperm count and motility. J. Med. Food in press (2007).Google Scholar
  13. Johannes, C. B., Araujo, A. B., Feldman, H. A., Derby, C. A., Kleinman, K. P., and McKinlay, J. B., Incidence of erectile dysfunction in men 40 to 69 years old; longitudinal results from the Massachusetts male aging study. J. Urol., 163, 460–463 (2000).PubMedCrossRefGoogle Scholar
  14. Jouannet, P., Wang, C., Eustache, F., Kold-Jensen, T., and Auger, J., Semen quality and male reproductive health: the controversy about human sperm concentration decline. Acta Pathol. Microbiol. Immunol. Scand., 109, 333–344 (2001).Google Scholar
  15. Juan, M. E., Gonzalez-Pons, E., Munuera, T., Ballester, J., Rodriguez-Gil, J. E., and Planas, J. M., trans-Resveratrol, a naturall antioxidant from grapes, increases sperm output in healthy rats. J. Nutr., 135, 757–760 (2005).PubMedGoogle Scholar
  16. Kleinert, H., Wallerath, T., Euchenhofer, C., Ihrig-Biedert, I., Li, H., and Förstermann, U., Estrogens increase transcription of the human endothelial NO synthase gene: analysis of the transcription factors involved. Hypertension, 31, 582–588 (1998).PubMedGoogle Scholar
  17. Li, H., Wallerath, T., and Förstermann, U., Physiological mechanisms regulating the expression of endothelial-type NO synthase. Nitric oxide Biol. Chem., 7, 103–118 (2002).CrossRefGoogle Scholar
  18. Padama-Nathan, H., Eardly, I., Kloner, R. A., Laties, A. M., and Montorsi, F., A 4 year update on the safety of sildenafil citrate (Viagra). Urology, 60(suppl. 2B), 67–90 (2002).CrossRefGoogle Scholar
  19. Sinclair, S., Male fertility: nutritional and environmental considerations. Altern. Med. Rev., 5, 28–38 (2005).Google Scholar
  20. Wallerath, T., Deckert, G., Ternes, T., Anderson, H., Li, H., Witte, K., and Förstermann, U., Resveratrol, a polyphenolic phytoalexin present in red wine, enhances expression and activity of endothelial nitric oxide synthase. Circulation, 106, 1652–1658 (2002).PubMedCrossRefGoogle Scholar
  21. Wallerath, T., Poleo, D., Li, H., and Forstermann, U., Red wine increase the expression of human endothelial nitric oxide synthase: a mechanism that may contribute to its beneficial cardiovascular effects. J. Am. College Cardiol., 41, 479–478 (2003).Google Scholar
  22. Wang, Z., Chen, Y., Lavinsky, N., Hsieh, T., Ungvari, Z., and Wu, J. M., Regulation of proliferation and gene expression in cultured human aortic smooth muscle cells by resveratrol and standardized grape extracts. Biochem. Biophys. Res. Commun., 346, 367–376 (2006).PubMedCrossRefGoogle Scholar

Copyright information

© The Pharmaceutical Society of Korea 2008

Authors and Affiliations

  • Sunhee Shin
    • 3
  • Jeong Hee Jeon
    • 3
  • Dongsun Park
    • 3
  • Min-Jung Jang
    • 3
  • Jae Hong Choi
    • 3
  • Bong-Ho Choi
    • 3
  • Seong Soo Joo
    • 3
  • Sang-Seop Nahm
    • 1
  • Jong-Choon Kim
    • 2
  • Yun-Bae Kim
    • 3
  1. 1.College of Veterinary MedicineKonkuk UniversitySeoulKorea
  2. 2.College of Veterinary MedicineChonnam National UniversityGwangjuKorea
  3. 3.College of Veterinary Medicine and Research Institute of Veterinary MedicineChungbuk National UniversityChungbukKorea

Personalised recommendations