Archives of Pharmacal Research

, Volume 31, Issue 1, pp 47–53 | Cite as

15,16-Dihydrotanshinone I, a major component from Salvia miltiorrhiza Bunge (Dansham), inhibits rabbit platelet aggregation by suppressing intracellular calcium mobilization

  • Jung-Woo Park
  • Sung-Hun Lee
  • Min-Kyu Yang
  • Jung-Jin Lee
  • Min-Jin Song
  • Si-Yong Ryu
  • Hun-Jong Chung
  • Hyung-Sik Won
  • Chang-Soo Lee
  • Suk-Hyung Kwon
  • Yeo-Pyo Yun
  • Wahn-Soo Choi
  • Hwa-Sup Shin
Article
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Abstract

Radix Salviae miltiorrhiza (RSM, ‘Dansham’ in Korea, ‘Danshen’ in Chinese), the root of Salviae miltiorrhiza Bunge (Labiate) has been used as Chinese fork medicine for the treatment of cardiovascular diseases such as angina pectoris, coronary heart disease, myocardial infarction, and hypertension. In the present study, we evaluated the inhibitory effects of 15, 16-Dihydrotanshinone I, one of the major ingredients of Salvia miltiorrhiza Bunge, on platelet aggregation, with elucidation of its mechanisms of action. 15,16-Dihydrotanshinone I concentration-dependently inhibited collagen-induced aggregation of rabbit washed platelets with IC50 of 8.7±5.6 μM, the potency being about seven-fold greater than EGCG, an active Green tea catechin component (IC50: 56.6±48.7 μM). 15,16-Dihydrotanshinone I significantly inhibited the intracellular calcium ([Ca2+]i) mobilization in a concentration-dependent manner. 15,16-dihdydrotanshinone I also significantly suppressed collagen (50 μg/mL)-induced liberation of [3H]Arachidonic acid from [3H]Arachidonic acid-incorporated rabbit platelet. In addition, 15,16-Dihydrotanshinone I at 50 μM slightly but significantly inhibited collagen-induced production of thromboxane B2. These results indicate that 15,16-Dihydrotanshinone I exert potent anti-platelet activity via suppression of [Ca2+]i mobilization and arachidonic acid liberation.

Key words

15,16-Dihydrotanshinone I Dansham Salviae Washed rabbit platelet Collagen Calcium 
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Copyright information

© The Pharmaceutical Society of Korea 2008

Authors and Affiliations

  • Jung-Woo Park
    • 7
  • Sung-Hun Lee
    • 7
  • Min-Kyu Yang
    • 7
  • Jung-Jin Lee
    • 1
  • Min-Jin Song
    • 7
  • Si-Yong Ryu
    • 2
  • Hun-Jong Chung
    • 3
  • Hyung-Sik Won
    • 4
  • Chang-Soo Lee
    • 7
  • Suk-Hyung Kwon
    • 5
  • Yeo-Pyo Yun
    • 1
  • Wahn-Soo Choi
    • 6
  • Hwa-Sup Shin
    • 7
  1. 1.College of PharmacyChungbuk National UniversityCheongjuKorea
  2. 2.Medicinal Chemistry Research TeamKorea Research Institute of Chemical TechnologyDaejeonKorea
  3. 3.Pediatric Department, Chungju Hospital, Konkuk Medical SchoolKonkuk UniversityChungjuKorea
  4. 4.Department of Biotehcnology, Division of Life Science, College of Biomedical and Health ScienceKonkuk UniversityChungju 380-701Korea
  5. 5.Rexgene Biotech Co. LtdSeoul 137-070Korea
  6. 6.College of MedicineKonkuk UniversityChungjuKorea
  7. 7.Department of Applied Biochemistry, Division of Life Science, College of Biomedical and Health ScienceKonkuk UniversityChungbukKorea

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