Archives of Pharmacal Research

, Volume 31, Issue 12, pp 1578–1583 | Cite as

Triterpenoic acids of Prunella vulgaris var. lilacina and their cytotoxic activities In Vitro

  • Il Kyun Lee
  • Do Hoon Kim
  • Seung Young Lee
  • Kyung Ran Kim
  • Sang Un Choi
  • Jong Ki Hong
  • Jei Hyun Lee
  • Young Hyun Park
  • Kang Ro Lee
Research Article Drug Efficacy and Safety

Abstract

The column chromatographic separation of the MeOH extract from the aerial parts of Prunella vulgaris var. lilacina Nakai led to the isolation of fifteen triterpenoic acids (2–6, 9–13, 16–20), four flavonoids (14, 21–23), four phenolics (7, 8, 15, 24), and a diterpene (1). Their structures were determined by spectroscopic methods to be trans-phytol (1), oleanic acid (2) ursolic acid (3), 2α,3α,19α-trihydroxyurs-12en-28oic acid (4), 2α,3α-dihydroxyurs-12en-28oic acid (5), maslinic acid (6), caffeic acid (7), phydroxy cinnamic acid (8), 2α,3α,19α,23-tetrahydroxyurs-12en-28oic acid (9), 2α,3α,23-trihydroxyurs-12en-28oic acid (10), 2α,3β-dihydroxyurs-12en-28oic acid (11), 2α,3β,24-trihydroxyolea-12en-28oic acid (12), (12R, 13S)-2α,3α,24,trihydroxy-12,13-cyclo-taraxer-14-en-28oic acid (13), quercertin 3-O-β-D-glucopyranoside (14), rosmarinic acid (15), 2α,3α,24-trihydroxyurs-12,20(30)-dien-28oic acid (16), 2α,3α,24-trihydroxyolea-12en-28oic acid (17), 2α,3β,19α,24-tetrahydroxyurs-12en-28oic acid 28-O-Dglucopyranoside (18), 2α,3α,19α,24-tetrahydroxyurs-12en-28oic acid 28-O-D-glucopyranoside (19), prunvuloside A (20), kaempferol 3-O-α-L-rhamnopyranosyl(1→6)-β-D-glucopranoside (21), kaempferol 3-O-β-D-glucopyranoside (22), quercertin 3-O-α-L-rhamnopyranosyl(1→6)-β-D-glucopyranoside (23), and 2-hydroxy-3-(3’,4’-dihydroxyphenly)propanoic acid (24). Compounds 1, 812, 17, 21, 23, and 24 were isolated from this plant source for the first time. The isolated compounds were evaluated for their cytotoxicity against A549, SK-OV-3, SK-MEL-2, and HCT15 cells in vitro using the sulforhodamin B bioassay (SRB) method. Compound 3 exhibited moderate cytotoxic activity against A549, SK-OV-3, SK-MEL-2, and HCT15 cells, with ED50 values of 3.71, 3.65, 13.62, and 5.44 μM, respectively.

Key words

Prunella vulgaris var. lilacina Triterpenoic acid Cytotoxicity 

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Copyright information

© The Pharmaceutical Society of Korea 2008

Authors and Affiliations

  • Il Kyun Lee
    • 1
  • Do Hoon Kim
    • 1
  • Seung Young Lee
    • 1
  • Kyung Ran Kim
    • 1
  • Sang Un Choi
    • 2
    • 1
  • Jong Ki Hong
    • 3
    • 1
  • Jei Hyun Lee
    • 4
    • 1
  • Young Hyun Park
    • 5
    • 1
  • Kang Ro Lee
    • 1
  1. 1.Natural Products Laboratory, College of PharmacySungkyunkwan UniversitySuwonKorea
  2. 2.Korea Research Institute of Chemical TechnologyTeajeonKorea
  3. 3.Pharmaceutical Analysis Laboratory, College of PharmacyKyung Hee UniversitySeoulKorea
  4. 4.College of Oriental MedicineDongguk UniversityGyeong juKorea
  5. 5.Department of Food Science and NutritionSoonchunhyang UniversityAsanKorea

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