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Archives of Pharmacal Research

, Volume 31, Issue 11, pp 1413–1418 | Cite as

Topoisomerase I and II inhibitory constituents from the bark of Tilia amurensis

  • Jae-Young Choi
  • Chang-Seob Seo
  • Ming-Shan Zheng
  • Chong-Soon Lee
  • Jong-Keun SonEmail author
Research Article Drug Efficacy and Safety

Abstract

Two coumarins (1 and 6), one flavan-3-ol (2), one fatty acid (3), and two lignan glycosides (4 and 5) were isolated from the EtOAc and CH2Cl2 extract of the bark of Tilia amurensis. Their chemical structures were identified by comparing their physicochemical and spectral data with those of published in literatures. Compounds 4, 5, and 6 were isolated from Tilia genus for the first time. Compounds 2 and 3 showed potent inhibitory activity against both DNA topoisomerase I (IC50 values; 49 μM and 4 μM, respectively, with 18 μM of positive control compound, comptothecin) and DNA topoisomerase II (IC50 values; 13 μM and 3 μM, respectively, with 50 μM of positive control compound, etoposide). However, all compounds did not showed cytotoxicity against the human colon adenocarcinoma cell line (HT-29), the human breast adenocarcinoma cell line (MCF-7), and human liver hepatoblastoma cell line (HepG-2).

Key words

Tilia amurensis Tiliaceae DNA topoisomerase I DNA topoisomerase II Cytotoxicity 

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Copyright information

© The Pharmaceutical Society of Korea 2008

Authors and Affiliations

  • Jae-Young Choi
    • 1
  • Chang-Seob Seo
    • 1
  • Ming-Shan Zheng
    • 1
  • Chong-Soon Lee
    • 2
    • 1
  • Jong-Keun Son
    • 1
    Email author
  1. 1.College of PharmacyYeungnam UniversityGyongsanKorea
  2. 2.Department of Biochemistry, College of ScienceYeungnam UniversityGyongsanKorea

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